Identification and Characterization of a Major Early-Transcribed Gene of Trichoplusia ni Single Nucleocapsid Nucleopolyhedrovirus Using the Baculovirus Expression System
Identification and Characterization of a Major Early-Transcribed Gene of Trichoplusia ni Single Nucleocapsid Nucleopolyhedrovirus Using the Baculovirus Expression System
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使用杆状病毒表达系统鉴定和表征粉纹夜蛾单核衣壳核多角体病毒的主要早期转录基因
DOI：
10.1023/b:viru.0000032786.45323.6b
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发表时间：
2004
期刊：
影响因子：
1.6
通讯作者：
P. Krell
中科院分区：
文献类型：
--
作者：
Weizhou Wang;S. Davison;P. Krell
测定并鉴定了新城疫霉单核衣壳核型多角体病毒(TnSNPV)南非株的一个早期转录基因(me-53)。它有一个1146个核苷酸的开放阅读框，编码一个382个氨基酸的蛋白质，相对分子质量为45.2 kDa。对13个杆状病毒ME-53S序列的同源性分析表明，TnSNPV ME-53与NPV亚群II-A甜菜夜蛾多重夜蛾和Mamestra Configurata(Maco)核型多角体病毒ME-53S同源性最高。ME-53S的C末端的锌指状基序高度保守，半胱氨酸的位置也很相似。将该基因和绿色荧光蛋白报告基因导入杆状病毒表达载体系统后，对两株感染链球菌核型多角体病毒(AcMNPV)的细胞株Me-53的转录分析表明，在感染后1h(HPI)至12HPI可检测到早期的TnSNPV Me-53转录本，在18HPI至48HPI可检测到晚期转录本，而重组AcMNPV Me-53的早期和晚期转录本分别可在感染后3HPI和24HPI检测到。提示AcMNPV和TnSNPV Me-53s的早期启动子和晚期启动子均在重组病毒感染细胞中被识别。对TnSNPV Me-53启动子的调控元件进行了分析。
An early transcribed gene (me-53) of a South Africa strain of Trichoplusia ni single nucleocapsid nucleopolyhedrovirus (TnSNPV) was sequenced and identified. It has an open reading frame of 1146 nucleotides that encodes a protein of 382 amino acids with a molecular mass of 45.2 kDa. The deduced protein sequence alignment of 13 baculovirus ME-53s indicated that the TnSNPV ME-53 shares the highest homologies with NPV subgroup II-A Spodoptera exigua multiple and Mamestra configurata (Maco) nucleopolyhedrovirus ME-53s. The zinc finger-like motifs at the C-termini of ME-53s are highly conserved with similar patterns of cysteine positions. Upon introduction of the gene and a green fluorescent protein reporter gene into the baculovirus expression vector system, the transcriptional analysis of me-53 in two cell lines infected with the Autographa californica nucleopolyhedrovirus (AcMNPV) recombinant revealed that an early TnSNPV me-53 transcript can be detected by 1 h postinfection (hpi) until 12 hpi and a late one from 18hpi up to 48 hpi, while early and late transcripts of the AcMNPV me-53 of the recombinant can be detected at 3 and 24 hpi, respectively. This suggested that the early and late promoters of both AcMNPV and TnSNPV me-53s were recognized in recombinant virus-infected cells. The regulatory elements of the TnSNPV me-53 promoter were also analyzed.
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影响因子：
3.7
作者：
BLISSARD, GW;KOGAN, PH;ROHRMANN, GF
通讯作者：
ROHRMANN, GF
影响因子：
3.7
作者：
BLISSARD, GW;ROHRMANN, GF
通讯作者：
ROHRMANN, GF
影响因子：
3.8
作者：
CHERBAS, L;CHERBAS, P
通讯作者：
CHERBAS, P
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S