ISOLATION, PURIFICATION, AND PARTIAL CHARACTERIZATION OF PRUNELLIN, AN ANTI-HIV COMPONENT FROM AQUEOUS EXTRACTS OF PRUNELLA-VULGARIS
ISOLATION, PURIFICATION, AND PARTIAL CHARACTERIZATION OF PRUNELLIN, AN ANTI-HIV COMPONENT FROM AQUEOUS EXTRACTS OF PRUNELLA-VULGARIS
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DOI：
10.1016/0166-3542(89)90036-3
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发表时间：
1989-06-01
影响因子：
7.6
通讯作者：
SMITH, KM
中科院分区：
文献类型：
--
作者：
TABBA, HD;CHANG, RS;SMITH, KM
夏枯草甙是从中药夏枯草的水提物中分离得到的一种具有抗HIV活性的化合物。红外光谱和核磁共振光谱鉴定prunellin为多糖。元素分析，沉淀与钙（II），钡（II），或9-氨基吖啶建议硫酸多糖。彻底水解材料的纸层析表明存在葡萄糖、半乳糖、木糖、葡萄糖酸、半乳糖酸和半乳糖胺作为组成单糖。通过凝胶渗透色谱法和在Sephadex G-75上的Squire方法测定，夏枯草素的分子大小约为10 kDa。
Prunellin, an anti-HIV active compound, was isolated from aqueous extracts of the Chinese medicinal herb, Prunella vulgaris, and purified to chromatographic homogeneity. Infrared and NMR spectroscopy identified prunellin as a polysaccharide. Elemental analyses, precipitation with calcium(II), barium(II), or 9-aminoacridine suggest a sulfated polysaccharide. Paper chromatography of the exhaustively hydrolyzed material indicates the presence of glucose, galactose, xylose, gluconic acid, galactonic acid and galactosamine as the constituent monosaccharides. The molecular size of prunellin, as determined by gel permeation chromatography and the Squire method on Sephadex G-75, is about 10 kDa.