Enveloped Virus Labeling via Both Intrinsic Biosynthesis and Metabolic Incorporation of Phospholipids in Host Cells
Enveloped Virus Labeling via Both Intrinsic Biosynthesis and Metabolic Incorporation of Phospholipids in Host Cells
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DOI：
10.1021/ac4008144
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发表时间：
2013-05-21
影响因子：
7.4
通讯作者：
Xie, Hai-Yan
中科院分区：
文献类型：
--
作者：
Huang, Li-Li;Lu, Gui-Hong;Xie, Hai-Yan
开发了一种用于标记完全复制型包膜病毒的替代方法，其中同时利用宿主细胞中磷脂的生物合成和代谢掺入，通过利用宿主衍生的脂质膜形成机制将叠氮基引入到牛痘病毒的包膜。这种叠氮基随后可用于通过生物正交反应荧光标记病毒的包膜。将这种包膜标记策略与病毒核酸的“复制-嵌入标记”相结合，巧妙地实现了病毒复制过程中的同时双标记。这是第一次，它是通过自然繁殖的病毒在其宿主细胞中的荧光团的存在下，同时双标记的活病毒可以条件。在简易温和的条件下高效温和地实现。
An alternative method for labeling fully replicative enveloped viruses was developed, in which both the biosynthesis and metabolic incorporation of phospholipids in host cells were simultaneously utilized to introduce an azide group to the envelope of the vaccinia virus by taking advantage of the host-derived lipid membrane formation mechanism. Such an azide group could be subsequently used to fluorescently label the envelope of the virus via a bioorthogonal reaction. Furthermore, simultaneous dual-labeling of the virus through the virus replication was realized skillfully by coupling this envelope labeling strategy with "replication-intercalation labeling" of viral nucleic acid. For the first time, it is by natural propagation of the virus in its host cells in the presence of fluorophores that simultaneous dual-labeling of living viruses can conditions. be mildly realized with high efficiency in facile and mild conditions.