Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli
Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli
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DOI：
10.1007/s00449-012-0821-2
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发表时间：
2013-07
影响因子：
3.8
通讯作者：
Si Jae Park;E. Y. Kim;Won Noh;Y. Oh;H. Kim;B. Song;K. Cho;S. Hong;S. H. Lee;J. Jegal
中科院分区：
文献类型：
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作者：
Si Jae Park;E. Y. Kim;Won Noh;Y. Oh;H. Kim;B. Song;K. Cho;S. Hong;S. H. Lee;J. Jegal
在这项研究中，我们开发了表达乳酸乳球菌亚杆菌的重组大肠杆菌。lactisIl1403谷氨酸脱羧酶（GadB）用于谷氨酸单钠盐（MSG）生产GABA。利用重组酶对味精合成GABA进行了研究。coliXL1-Blue作为缓冲溶液中的全细胞生物催化剂。通过增加e的浓度。从2-10的od600中表达gabb的coliXL1-Blue， 10 g/L味精产生的GABA浓度和转化率分别从4.3 g/L和70%提高到4.8 g/L和78%。此外,E。表达GadB的coliXL1-Blue在200 g/L味精中产生76.2 g/L的GABA， GABA产量为62.4%。最后，以微生物合成的GABA为原料，以al2o3为催化剂，在甲苯中反应得到2-吡咯烷酮，采用本体聚合法制备了尼龙4，收率达96%。
In this study, we developed recombinantEscherichia colistrains expressingLactococcus lactissubsp.lactisIl1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinantE. coliXL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration ofE. coliXL1-Blue expressing GadB from the OD600of 2–10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore,E. coliXL1-Blue expressing GadB highly concentrated to the OD600of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al2O3as catalyst in toluene with the yield of 96 %.