Highly fluorescent streptavidin-coated CdSe nanoparticles:: Preparation in water, characterization, and micropatterning
Highly fluorescent streptavidin-coated CdSe nanoparticles:: Preparation in water, characterization, and micropatterning
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DOI：
10.1021/la0363940
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发表时间：
2004-05-11
期刊：
影响因子：
3.9
通讯作者：
Vogel, H
中科院分区：
文献类型：
--
作者：
B채umle, M;Stamou, D;Vogel, H
以谷胱甘肽为稳定剂，在水中一步合成了具有25%的高荧光量子产率和窄尺寸分布的CdSe量子点。这种特殊的光学性质首次使人们能够在室温下检测水中制备的单量子点。对于作为荧光生物分析探针的应用，QD用链霉亲和素包被。这些量子点在微图案化生物素上以高对比度自组装，同时保留其光学性质和结合生物素化分子的能力，这是开发新型超分子结构和生物测定的先决条件。
CdSe quantum dots (QDs) with a high fluorescence quantum yield of 25% and a narrow size distribution were synthesized in a single step in water using glutathione as a stabilizing molecule. The exceptional optical properties enabled for the first time the detection of in-water-prepared single quantum dots at room temperature. For application as fluorescent bioanalytical probes, the QDs were coated with streptavidin. These QDs self-assemble with high contrast on micropatterned biotin while preserving their optical properties and their capability to bind in addition biotinylated molecules, a prerequisite for the development of novel supramolecular structures and bioassays.