Ab Initio Determination of Peptide Structures by MicroED.
Ab Initio Determination of Peptide Structures by MicroED.
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DOI：
10.1007/978-1-0716-0966-8_17
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Rodriguez JA
中科院分区：
文献类型：
--
作者：
Zee CT;Saha A;Sawaya MR;Rodriguez JA
近年来，随着越来越多的技术进步，现有的晶体学方法已经促进了小分子（如肽）的结构解析。在低温条件下蛋白质纳米晶体的电子显微衍射（MicroED）的出现使得只有数百纳米厚的结晶肽组装体的询问成为可能。通过这些方法收集原子或近原子分辨率的数据，允许从头确定各种淀粉样蛋白形成肽的结构，包括来自朊病毒和冰成核蛋白的片段。本章重点介绍了从纳米级肽组装体和其他类似分子的从头计算结构确定的过程。
Structural elucidation of small macromolecules such as peptides has recently been facilitated by a growing number of technological advances to existing crystallographic methods. The emergence of electron micro-diffraction (MicroED) of protein nanocrystals under cryogenic conditions has enabled the interrogation of crystalline peptide assemblies only hundreds of nanometers thick. Collection of atomic or near-atomic resolution data by these methods has permitted the ab initio determination of structures of various amyloid-forming peptides, including segments derived from prions and ice-nucleating proteins. This chapter focuses on the process of ab initio structural determination from nano-scale peptide assemblies and other similar molecules.