The expression of IL-20 and IL-24 and their shared receptors are increased in rheumatoid arthritis and spondyloarthropathy
The expression of IL-20 and IL-24 and their shared receptors are increased in rheumatoid arthritis and spondyloarthropathy
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DOI：
10.1016/j.cyto.2007.10.004
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发表时间：
2008-01-01
期刊：
影响因子：
3.8
通讯作者：
Deleuran, Bent
中科院分区：
文献类型：
--
作者：
Kragstrup, Tue Wenzel;Otkjaer, Kristian;Deleuran, Bent
本研究的目的是分析两种促炎细胞因子IL-20和IL-24及其共享受体在类风湿关节炎和脊柱关节病中的表达。与骨关节炎患者相比，类风湿性关节炎患者血浆中的IL-20增加，与骨关节炎患者相比，类风湿性关节炎和脊柱关节病患者的滑液和血浆中的IL-24增加。IL-20和IL-24 mRNA仅以低水平存在于滑膜中。在滑膜中，IL-20蛋白存在于单核细胞和中性粒细胞中，而IL-24蛋白存在于内皮细胞和单核细胞中。IL-20受体I型和IL-22受体由滑液中的粒细胞表达。在滑液单核细胞培养物中，重组人IL-20或重组人IL-24刺激诱导单核细胞趋化蛋白I（CCL 2/MCP-1）分泌，但不诱导肿瘤坏死因子α mRNA合成或IL-6分泌。IL-20和IL-24均与类风湿关节炎和脊柱关节病患者血浆中的CCL 2/MCP-1相关。本研究将IL-20和IL-24与类风湿性关节炎和脊柱关节病的滑膜相关联，结果表明这两种细胞因子通过募集中性粒细胞和诱导CCL 2/MCP-1而促进疾病发病机制。(c)2007爱思唯尔有限公司保留所有权利。
The purpose of this study was to analyze the expression of the two proinflammatory cytokines IL-20 and IL-24 and their shared receptors in rheumatoid arthritis and spondyloarthropathy. IL-20 was increased in plasma of rheumatoid arthritis patients compared with osteoarthritis patients and IL-24 was increased in synovial fluid and plasma of rheumatoid arthritis and spondyloarthropathy patients compared with osteoarthritis patients. IL-20 and IL-24 mRNA was only present at low levels in the synovium. In the synovial membrane, IL-20 protein was present in mononuclear cells and neutrophil granulocytes whereas IL-24 protein was observed in endothelial cells and mononuclear cells. IL-20 receptor type I and IL-22 receptor were expressed by granulocytes in the synovial fluid. In synovial fluid mononuclear cell cultures, stimulation with recombinant human IL-20 or recombinant human IL-24 induced monocyte chemoattractant protein I (CCL2/MCP-1) secretion, but not tumour necrosis factor alpha mRNA synthesis or IL-6 secretion. Both IL-20 and IL-24 showed correlations to CCL2/MCP-1 in plasma from rheumatoid arthritis and spondyloarthropathy patients. This study associates IL-20 and IL-24 to the synovium of rheumatoid arthritis and spondyloarthropathy and results indicate that the two cytokines contribute to disease pathogenesis through recruitment of neutrophil granulocytes and induction of CCL2/MCP-1. (c) 2007 Elsevier Ltd. All rights reserved.