The mitochondrial genome of Qinghuang_1, the first modern improved strain of Chinese oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)
The mitochondrial genome of Qinghuang_1, the first modern improved strain of Chinese oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)
复制标题
柞蚕现代第一个改良品系青黄_1的线粒体基因组（鳞翅目：蚕科）
DOI：
10.3920/jiff2020.0054
复制
发表时间：
2021-01-01
影响因子：
5.1
通讯作者：
Qin, L.
中科院分区：
文献类型：
--
作者：
Li, X-Y;Liu, Y-C;Qin, L.
柞蚕（Antheraea pernyi Guerin-Meneville 1855）是我国传统的食用昆虫，也是最具发展潜力的食用昆虫。目前还没有关于该家蚕的第一个现代改良品系青黄_1的线粒体基因组（有丝分裂基因组）的信息。在此，我们通过长PCR扩增和Illumina测序确定了青黄_1的有丝分裂基因组，然后将所得的有丝分裂基因组与该物种的五个可用的有丝分裂基因组进行比较。青黄_1的有丝分裂基因组长度为15，573 bp，与已知的A. pernyi有丝分裂基因组。该有丝分裂基因组的碱基A含量高于其他四个菌株，但低于野生型。序列比较发现，5个近交系中有200个单核苷酸变异（1.28%）和32个氨基酸变化，表明A.柞蚕种质资源ND 1的3'端是A. pernyi mitogenome. Ka/Ks分析表明，除ND 5基因的Ka/Ks值大于1外，其余蛋白质编码基因均在负选择条件下进化，表明该基因可能受到正选择的作用。系统发育分析证实了青黄_1在A. pernyi。我们的研究结果表明，有丝分裂基因组有助于理解种内系统发育关系。pernyi及其遗传改良。
Chinese oak silkworm, Antheraea pernyi Guerin-Meneville 1855 (Lepidoptera: Saturniidae), is a traditional edible insect in China and is considered the edible insect with the highest potential. Information on the mitochondrial genome (mitogenome) of the first modern improved strain of this silkworm, Qinghuang_1, is currently unavailable. Here, we determine the mitogenome of Qinghuang_1 by long PCR amplification followed by Illumina sequencing and then compare the resulting mitogenome with the five available mitogenomes of this species. The mitogenome of Qinghuang_1 is 15,573 bp in length and exhibits an identical gene organisation to known A. pernyi mitogenomes. The base A content of this mitogenome is higher than those of the other four strains but lower than that of the wild type. Sequence comparisons identified 200 single-nucleotide variants (1.28%) and 32 amino acid changes among the five inbred strains, indicating a considerable degree of nucleotide diversity in the mitogenomes of A. pernyi germplasm resources. The 3' end of ND1 was identified as a hotspot in the A. pernyi mitogenome. Ka/Ks analysis indicated that all protein-coding genes evolved under negative selection except for ND5, which presented values larger than 1, suggesting that positive selection may act on this gene. The phylogenetic analyses confirmed the basal position of Qinghuang_1 among the inbred strains of A. pernyi. Our results indicated that the mitogenome is helpful for understanding the intraspecific phylogenetic relationships of A. pernyi and for its genetic improvement.