Identification of quantitative trait loci controlling rice mature seed culturability using chromosomal segment substitution lines
Identification of quantitative trait loci controlling rice mature seed culturability using chromosomal segment substitution lines
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DOI：
10.1007/s00299-008-0641-7
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发表时间：
2009-02-01
影响因子：
6.2
通讯作者：
Yu, Sibin
中科院分区：
文献类型：
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作者：
Zhao, Lina;Zhou, Hongju;Yu, Sibin
水稻品种的遗传转化效率在很大程度上取决于其组织培养能力。高效组织培养体系的建立，极大地促进了转基因粳稻品种的推广应用。然而，由于籼稻品种对离体培养有抗性，这一过程受到了阻碍。利用籼稻品种“珍山97B”与粳稻品种“日本裸子”杂交的染色体片段代换系（CSSL）群体，对成熟种子可培养性的数量性状位点（qtl）进行了定位。CSSLs由139个品系组成，每个品系包含一个或几个渗入片段，它们共同覆盖了整个Nipponbare基因组。每个CSSL分别在籼稻亲本品种和粳稻亲本品种开发的两种培养基体系上进行了培养试验。通过愈伤组织诱导频率（CIF）、愈伤组织继代培养能力（CSC）、植株再生频率（PRF）和每再生愈伤组织平均苗数（MNR） 4项指标评价其培养性能。这4个性状在CSSLs中均表现为连续变异。在粳稻培养体系中，检测到3个CIF qtl、3个CSC qtl、3个PRF qtl和3个MNR qtl。在籼稻品种培养体系中，鉴定出6个CIF qtl、2个CSC qtl、3个PRF qtl和6个MNR qtl，这些qtl分布在9条水稻染色体上。在粳稻和籼稻栽培体系中均检测到2个CIF和2个MNR qtl。四种性状的相关系数随培养体系的不同而不同。这些结果为利用粳稻所需等位基因进行标记辅助育种提高籼稻的可育性提供了可能。
The genetic transformation efficiency of a rice variety is largely determined by its tissue culturability. Establishment of a highly efficient tissue-culture system has greatly accelerated the wide spread application of transgenic japonica varieties. However, such process for indica rice was hampered because this type of variety is recalcitrant to in vitro culture. This study aimed to map the quantitative trait loci (QTLs) for mature seed culturability using a chromosomal segment substitution lines (CSSL) population derived from a cross between an indica variety "Zhenshan 97B" and a japonica variety "Nipponbare". The CSSLs consist of 139 lines each containing a single or a few introgression segments, and together covering the whole "Nipponbare" genome. Every CSSL was tested by culturing on the two medium systems developed for the respective indica and japonica parental varieties. The performance of culturability was evaluated by four indices: frequency of callus induction (CIF), callus subculture capability (CSC), frequency of plant regeneration (PRF) and the mean plantlet number per regenerated callus (MNR). All four traits displayed continuous variation among the CSSLs. With the culture system for japonica rice, three CIF QTLs, three CSC QTLs, three PRF QTLs and three MNR QTLs were detected. With the culture system for indica variety, six CIF QTLs, two CSC QTLs, three PRF QTLs and six MNR QTLs were identified, and these QTLs distributed on nine rice chromosomes. Two QTLs of CIF and two QTLs of MNR were detected in both the japonica and indica rice culture system. The correlation coefficients of all the four traits varied depending on the culture systems. These results provide the possibilities of enhancing the culturability of indica rice by marker-assisted breeding with those desirable alleles from the japonica.