The periodontopathic bacteria in placenta, saliva and subgingival plaque of threatened preterm labor and preterm low birth weight cases: a longitudinal study in Japanese pregnant women
The periodontopathic bacteria in placenta, saliva and subgingival plaque of threatened preterm labor and preterm low birth weight cases: a longitudinal study in Japanese pregnant women
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DOI：
10.1007/s00784-020-03287-4
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发表时间：
2020-04
影响因子：
3.4
通讯作者：
C. Ye;Sayaka Katagiri;N. Miyasaka;H. Kobayashi;Thatawee Khemwong;T. Nagasawa;Y. Izumi
中科院分区：
文献类型：
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作者：
C. Ye;Sayaka Katagiri;N. Miyasaka;H. Kobayashi;Thatawee Khemwong;T. Nagasawa;Y. Izumi
目的本研究通过检测先兆早产（TPL）和早产低出生体重（PLBW）孕妇的唾液、龈下菌斑和胎盘中牙周致病菌的数量，以确定与不良妊娠结局高度相关的牙周致病菌。齿垢密螺旋体，具核梭杆菌，和中间普氏菌在龈下菌斑，唾液，胎盘组织，除了血清IgG滴度对这些细菌在28例TPL和36名健康孕妇women.ResultsThirteen 64分娩PLBW婴儿。6种牙周致病菌均在胎盘标本中检出。F. T. TPL组胎盘样本中的齿状突蛋白显著高于健康组。年龄、血清抗P. gingivalIgG、P. gingivalisandT.在菌斑样本中，P. TPL-PLBW组的唾液中唾液分泌量、牙周袋探诊深度≥ 5 mm的百分比高于TPL-HD组和/或H-HD组。有序Logistic回归分析显示F. TPL中胎盘组织中的核瘤与TPL显著相关，而母亲年龄与TPL中的PLBW显著相关。F.结论：TPL的发生可能与胎盘中的核毒素有关，而高龄产妇的TPL发生可能与PLBW有关。
ObjectivesThis study determined the quantity of periodontopathic bacteria in saliva, subgingival plaque, and placenta on the threatened preterm labor (TPL) and preterm low birth weight (PLBW) subjects in order to identify specific periodontal pathogens with high association to adverse pregnancy outcomes.MethodsWe used real-time PCR with TaqMan probe and ELISA to detect the amount ofAggregatibacter actinomycetemcomitans,Porphyromonas gingivalis,Tannerella forsythia,Treponema denticola,Fusobacterium nucleatum, andPrevotella intermediain subgingival plaque, saliva, and placenta tissue, in addition to serum IgG titers against these bacteria in 28 patients with TPL and 36 healthy pregnant women.ResultsThirteen of 64 births delivered PLBW infants. All 6 periodontopathic bacteria were detected in the placenta samples. The amount ofF. nucleatumand detection frequency ofT. denticolain placental samples was significantly higher in the TPL group than in the healthy group. Meanwhile, the age, anti-P. gingivalIgG in serum, amount ofP. gingivalisandT. forsythiain plaque samples, detection frequency ofP. intermediain saliva, and percentage of pocket probing depth ≥ 5 mm were higher in TPL-PLBW births than those in TPL-Healthy delivery (HD) group and/or in H-HD group. Ordinal logistic regression analysis revealed that the presence ofF. nucleatumin placental tissues was significantly associated with TPL, while the maternal age was significantly associated with PLBW in TPL.ConclusionOur findings suggested all 6 bacteria may access the placenta. The increased presence ofF. nucleatumin placenta might be related to TPL, while advanced maternal age might be associated with PLBW in TPL.Clinical relevancePeriodontal therapy should be applied to reduce the deep periodontal pocket sites and the colonization of periodontal pathogens in high-risk population.