Proportion regulation without pattern formation in Dictyostelium discoideum.
Proportion regulation without pattern formation in Dictyostelium discoideum.
复制标题
盘基网柄菌中不形成图案的比例调节。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
I. Takeuchi
中科院分区：
文献类型：
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作者：
M. Oyama;K. Okamoto;I. Takeuchi
当在葡萄糖-白蛋白-cAMP培养基中摇动时，解离的聚集细胞形成小团块，其中前孢子和前柄细胞以与蛞蝓基本相同的比例分化。免疫细胞化学染色显示，这两种类型的细胞没有显示出特定的分布模式，不像在蛞蝓中观察到的两个分区prestalk-prepore模式。细胞解离的阶段晚于发病的聚集总是产生一个恒定比例的前孢子细胞，无论初始比例时，转移到培养。此外，从蛞蝓分离的前柄细胞转移到培养物中，通过细胞类型的转换，几乎恢复了正常的前孢子比例，而未分离的蛞蝓细胞的比例保持不变。我们得出结论，从这些发现，正常的prestalk-prepore模式是不需要比例进行调节。
When shaken in a glucose-albumin-cAMP medium, dissociated aggregative cells formed small clumps, in which both prespore and prestalk cells differentiated in essentially the same proportions as in a slug. Immunocytochemical staining of sections of such clumps revealed that the two types of cells showed no particular pattern of distribution, unlike the two-zoned prestalk-prespore pattern as observed in the slug. Cells dissociated at stages later than the onset of aggregation always produced a constant proportion of prespore cells, irrespective of the initial proportion when transferred to the culture. Furthermore, prestalk cells fractionated from slugs and transferred to the culture restored almost the normal prespore protortion through conversion of the cell types, whereas proportion of unfractionated slug cells remained unchanged. We conclude from these findings that the normal prestalk-prespore pattern is not required for proportion to be regulated.