17β-Estradiol is critical for the preovulatory induction of prostaglandin E2 synthesis in mice.
17β-Estradiol is critical for the preovulatory induction of prostaglandin E2 synthesis in mice.
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17β-雌二醇对于小鼠排卵前诱导前列腺素 E2 合成至关重要。
DOI：
10.1016/j.mce.2012.06.006
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发表时间：
2012
影响因子：
4.1
通讯作者：
and Saibara T.
中科院分区：
文献类型：
--
作者：
Toda K;Ono M;Yuhki K;Ushikubi F;and Saibara T.
芳香化酶缺陷（ArKO）小鼠由于雌激素产生不足而完全无排卵。然而，连续给予高剂量的17β-雌二醇（E2）和促性腺激素可诱导这些小鼠排卵。由于前列腺素E2（PGE 2）是诱导排卵的关键介质之一，因此，我们研究了排卵刺激对ArKO小鼠前列腺素E2代谢相关基因表达和卵巢PGE 2含量的影响。排卵刺激显著增加排卵前ArKO卵巢中前列腺素内过氧化物合成酶2、前列腺素E2受体4型和磺基转移酶家族1 E成员1的mRNA表达。相反，它抑制15-羟基前列腺素脱氢酶的mRNA表达。此外，在排卵前卵巢的ArKO小鼠的前列腺素E 2含量显着升高后，检测到与E2刺激排卵剂量的促性腺激素。因此，这些分析表明，排卵前需要E2促进PGE 2的合成，从而导致未来排卵的成功。
Aromatase-deficient (ArKO) mice are totally anovulatory due to insufficient estrogen production. However, sequential administrations of high doses of 17β-estradiol (E2) and gonadotropins were found to induce ovulation in these mice. Here, we examined how the ovulatory stimulation for ArKO mice alters the expressions of genes related to prostaglandin (PG) E2metabolism and ovarian contents of PGE2, as PGE2is one of the critical mediators of ovulatory induction. The ovulatory stimulation significantly increased mRNA expressions of prostaglandin-endoperoxide synthase 2, PGE2receptor type 4 and sulfotransferase family 1E, member 1, in preovulatory ArKO ovaries. In contrast, it suppressed the mRNA expression of 15-hydroxyprostaglandin dehydrogenase. Furthermore, significant elevation in the PGE2contents was detected in the preovulatory ovaries of ArKO mice after stimulation with E2 plus ovulatory doses of gonadotropins. Thus, these analyses demonstrate a requirement of E2 for the preovulatory enhancement of PGE2synthesis, leading to future success in ovulation.