Molecular analysis of β-proteobacterial ammonia oxidizer populations in surface layers of a submerged paddy soil microcosm
Molecular analysis of β-proteobacterial ammonia oxidizer populations in surface layers of a submerged paddy soil microcosm
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DOI：
10.1080/00380768.2003.10410355
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发表时间：
2003-12
影响因子：
2
通讯作者：
J. Murase;K. Itoh;Mayuko Kana;M. Kimura
中科院分区：
文献类型：
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作者：
J. Murase;K. Itoh;Mayuko Kana;M. Kimura
摘要 通过对使用 AOB 选择性引物扩增的 16S rRNA 基因片段进行变性梯度凝胶电泳 (DGGE) 分析，确定了沉水稻田微观世界中 β-变形菌自养氨氧化细菌 (AOB) 群落的结构。淹没后 4 周观察到群落组成的变化。土壤微观世界表层（0-1、2-3 mm）的群落与地下层（6-9、> 15 mm）的群落不同，检测到各层特有的 DGGE 带。大多数检索到的序列是亚硝化螺菌样序列，而没有获得亚硝化单胞菌样序列。 16S rDNA 引物组还扩增了与已知的亚硝化螺菌-亚硝化单胞菌群无关的序列，尽管它们显示出与其他β-变形菌群密切相关。结果表明，类亚硝化螺菌种群是沉水水稻土中AOB的优势种群，沉水水稻土的氧化层中存在着特定的AOB。
Abstract The structure of the β-proteobacterial autotrophic ammonia-oxidizing bacterial (AOB) communities in a microcosm of submerged paddy soil was determined by denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments amplified using AOB-selective primers. Shift in the community composition was observed 4 weeks after submergence. The communities from the surface layers (0–1, 2–3 mm) of the soil microcosm were different from those of the subsurface layers (6–9, > 15 mm) and DGGE bands specific to each layer were detected. The majority of the retrieved sequences were Nitrosospira-like, whereas no Nitrosomonas-like sequences were obtained. The 16S rDNA primer set also amplified sequences that were not related to the known Nitrosospira-Nitrosomonas group, although they showed a close relationship with other groups of β-proteobacteria. The results suggest that Nitrosospira-like populations are dominant AOB populations in the submerged paddy soil, and that the oxic layer of submerged paddy soil harbours the specific AOB.