Long-term survival of the mouse ES cell-derived mast cell, MEDMC-BRC6, in mast cell-deficient KitW-sh/W-sh mice
Long-term survival of the mouse ES cell-derived mast cell, MEDMC-BRC6, in mast cell-deficient KitW-sh/W-sh mice
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小鼠 ES 细胞衍生的肥大细胞 MEDMC-BRC6 在肥大细胞缺陷 KitW-sh/W-sh 小鼠中的长期存活
DOI：
10.1093/intimm/dxx022
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发表时间：
2017
影响因子：
4.4
通讯作者：
Shibuya Akira
中科院分区：
文献类型：
--
作者：
Shibagaki Shohei;Tahara-Hanaoka Satoko;Hiroyama Takashi;Nakamura Yukio;Shibuya Akira
肥大细胞（MCs）通过ige依赖性和非依赖性信号通路在过敏反应和宿主对微生物感染的防御中发挥关键作用。能够在体外和体内分析的MC细胞系将有助于MC依赖性免疫应答的研究。在这里，我们研究了小鼠胚胎干细胞衍生的mc样细胞系MEDMC-BRC6的功能特征。该细胞系表达FcεRI和c-Kit，并在FcεRI与IgE交联后表现出脱颗粒和炎症细胞因子和趋化因子的产生，包括TNF-α、IL-6和MCP-1。这些细胞因子和趋化因子也通过TLR2和TLR4的刺激在细胞系中产生。将MEDMC-BRC6移植到基因兼容的mc -缺陷kitw -sh/W-shmice中，成功诱导全身过敏反应后，MEDMC-BRC6在腹腔和耳部皮肤中存活至少6个月。因此，MEDMC-BRC6细胞是研究体外和体内MCsin功能的有效工具。
Mast cells (MCs) play pivotal roles in allergic reactions and the host defense against microbial infection through the IgE-dependent and IgE-independent signaling pathways. MC lines that can be analyzed bothin vitroandin vivowould be useful for the study of MC-dependent immune responses. Here, we investigated the functional characteristics of a mouse embryonic stem cell-derived MC-like cell line, MEDMC-BRC6. The cell line expressed FcεRI and c-Kit and showed degranulation and production of inflammatory cytokines and chemokines, including TNF-α, IL-6 and MCP-1, upon cross-linking FcεRI with IgE. These cytokines and chemokines were also produced by the cell line by stimulation of TLR2 and TLR4. MEDMC-BRC6 survived in the peritoneal cavity and the ear skin for at least 6 months after the transfer into genetically compatible MC-deficientKitW-sh/W-shmice, in which systemic anaphylaxis was successfully induced. Thus, MEDMC-BRC6 cells represent a potent tool for investigating the functions of MCsin vitroandin vivo.