Surface roughness of silk fibroin/alginate microspheres for rapid hemostasis in vitro and in vivo
Surface roughness of silk fibroin/alginate microspheres for rapid hemostasis in vitro and in vivo
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用于体外和体内快速止血的丝素蛋白/海藻酸盐微球的表面粗糙度
DOI：
10.1016/j.carbpol.2020.117256
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发表时间：
2021-02-01
影响因子：
11.2
通讯作者：
Chen, Guobao
中科院分区：
文献类型：
--
作者：
Huang, Xiang;Fu, Qiang;Chen, Guobao
及时止血治疗对于减少失血、提高生存率非常重要。为了提高血液凝固速度，通过海藻酸钠（SA）和丝素蛋白（SF）的乳液交联制备了不同程度表面粗糙度的止血微球。通过扫描电子显微镜（SEM）、红外光谱和凝血实验分析表面形貌和止血性能。结果表明，当SA与SF的体积比为2:1（SF/SA2）时，微球表面最粗糙。这些微球聚集的红细胞数量最多、凝血速度最快、凝血强度最强。微球不仅无细胞毒性，而且还能促进细胞生长。体外和体内凝血实验表明，SF/SA2微球可以减少出血时间和出血量，提高止血效率，这表明SF/SA2微球是具有良好生物安全性的潜在止血剂。
Timely hemostatic treatment is important to reduce blood loss and improve survival. To increase the speed of blood coagulation, hemostatic microspheres with varying degrees of surface roughness were prepared by emulsion cross-linking of sodium alginate (SA) and silk fibroin (SF). Surface morphology and hemostatic properties were analyzed with scanning electron microscope (SEM), infrared spectroscopy, and blood coagulation experiments. The results showed that microsphere have the roughest surface when the volume ratio of SA to SF is 2:1 (SF/SA2). These microspheres have the largest number of aggregated red blood cells, the fastest coagulation rates, and the strongest coagulation strength. Not only microspheres are they non-cytotoxic but they also promote cell growth. In vitro and in vivo coagulation experiments demonstrated that SF/SA2 microspheres can reduce bleeding time and volume and improve hemostatic efficiency, which suggests that SF/SA2 microspheres are potential hemostatic agents equipped with excellentbiosecurity.