Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19.
Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19.
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DOI：
10.3390/ijms22052723
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发表时间：
2021-03-08
影响因子：
5.6
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Tian L;Elsheikh EB;Patrone PN;Kearsley AJ;Gaigalas AK;Inwood S;Lin-Gibson S;Esposito D;Wang L
定量和可靠的血清学分析是支撑全球新冠肺炎对诊断、监测和疫苗开发反应的关键衡量标准。在这里，我们报告了一种概念验证方法，用于开发基于多重流式细胞术的定量血清学和中和分析。血清学检测针对全长刺突抗原和刺突抗原受体结合域(RBD)的免疫球蛋白和免疫球蛋白。以RBD特异性SARS-CoV免疫球蛋白为参照标准，将抗SARS-CoV-2 RBD抗体滴度量化在37.6微克/毫升至31.0毫微克/毫升之间。定量检测具有较高的特异性，与MERS、SARS1、OC43和HKU1病毒的刺突蛋白无交叉反应。我们进一步证明了抗RBD抗体效价和中和抗体效价之间具有良好的相关性。这套血清学和中和分析有助于提高测量的可信度，是临床和流行病学研究的补充和基础。
Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow cytometry-based serological and neutralization assays. The serology assays test the IgG and IgM against both the full-length spike antigens and the receptor binding domain (RBD) of the spike antigen. Benchmarking against an RBD-specific SARS-CoV IgG reference standard, the anti-SARS-CoV-2 RBD antibody titer was quantified in the range of 37.6 µg/mL to 31.0 ng/mL. The quantitative assays are highly specific with no correlative cross-reactivity with the spike proteins of MERS, SARS1, OC43 and HKU1 viruses. We further demonstrated good correlation between anti-RBD antibody titers and neutralizing antibody titers. The suite of serology and neutralization assays help to improve measurement confidence and are complementary and foundational for clinical and epidemiologic studies.
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高度敏感和特异性的多重抗体测定法对SARS-COV-2抗原量化免疫球蛋白M，A和G。
影响因子：
9.4
作者：
Dobaño C;Vidal M;Santano R;Jiménez A;Chi J;Barrios D;Ruiz-Olalla G;Rodrigo Melero N;Carolis C;Parras D;Serra P;Martínez de Aguirre P;Carmona-Torre F;Reina G;Santamaria P;Mayor A;García-Basteiro AL;Izquierdo L;Aguilar R;Moncunill G
通讯作者：
Moncunill G
影响因子：
8
作者：
Abe, Kento T.;Li, Zhijie;Gingras, Anne-Claude
通讯作者：
Gingras, Anne-Claude
影响因子：
30.3
作者：
Case, James Brett;Rothlauf, Paul W.;Whelan, Sean P. J.
通讯作者：
Whelan, Sean P. J.
影响因子：
4.6
作者：
Di Veroli GY;Fornari C;Goldlust I;Mills G;Koh SB;Bramhall JL;Richards FM;Jodrell DI
通讯作者：
Jodrell DI
影响因子：
4.2
作者：
Gundlapalli AV;Salerno RM;Brooks JT;Averhoff F;Petersen LR;McDonald LC;Iademarco MF;CDC COVID-19 Response
通讯作者：
CDC COVID-19 Response