Engineering resistance against tomato yellow leaf curl virus (TYLCV) using antisense RNA
Engineering resistance against tomato yellow leaf curl virus (TYLCV) using antisense RNA
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DOI：
10.1023/a:1005715805000
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发表时间：
1997-01-01
影响因子：
5.1
通讯作者：
Gronenborn, B
中科院分区：
文献类型：
--
作者：
Bendahmane, M;Gronenborn, B
番茄黄化曲叶病毒(TYLCV)是由烟粉虱传播的一种双生病毒，是世界范围内栽培番茄最严重的病害之一。在这里，我们描述了反义RNA在干预TYLCV引起的疾病中的应用。反义RNA的靶标是由c1基因编码的Rep蛋白的稀有信使RNA。获得了表达C1反义RNA的转基因烟草植株，并证明其能抵抗TYLCV的侵染。部分抗性品系无症状，TYLCV的复制几乎完全被抑制。转基因介导的抗性在至少两代子代中被证明是有效的。
One of the most severe diseases of cultivated tomato worldwide is caused by tomato yellow leaf curl virus (TYLCV), a geminivirus transmitted by the whitefly Bemisia tabaci. Here we describe the application of antisense RNAs to interfere with the disease caused by TYLCV. The target of the antisense RNA is the rare messenger RNA of the Rep protein, encoded by the C1 gene. Transgenic Nicotiana benthamiana plants expressing C1 antisense RNA were obtained and shown to resist infection by TYLCV. Some of the resistant lines are symptomless, and the replication of challenge TYLCV almost completely suppressed. The transgenes mediating resistance were shown to be effective through at least two generations of progeny.