Steroid hormone receptor status of mouse mammary stem cells
Steroid hormone receptor status of mouse mammary stem cells
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DOI：
10.1093/jnci/djj267
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发表时间：
2006-07-19
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影响因子：
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通讯作者：
Lindeman, Geoffrey J.
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作者：
Asselin-Labat, Marie-Liesse;Shackleton, Mark;Lindeman, Geoffrey J.
雌激素受体α（ER α）、孕激素受体（PR）和erbB 2（人类Her 2）是人类乳腺癌的重要预后标志物，并且它们在不同亚型的乳腺癌中不同程度地表达。例如，基底亚型通过免疫组织化学对ER a、PR和Her 2呈阴性。我们研究了这些信号分子在小鼠乳腺干细胞和管腔细胞的富集群体中的表达，这些细胞是根据其CD 24和α 6 β 1-整合素复合物的差异表达分离的。我们发现富含小鼠乳腺干细胞的基础细胞群不表达ERa、PR或ErbB 2/Her 2，但表达表皮生长因子受体（EGFR）/ErbB 1，而富含管腔细胞的细胞亚群表达ERa（37%的细胞）和PR（40%的细胞），但不表达ErbB 2/Her 2或EGFR/ErbB 1。卵巢切除术证实了雌激素信号对管腔细胞增殖的重要性，但对小鼠乳腺干细胞富集群体的大小没有影响。因此，小鼠乳腺干细胞对ER α、PR和ErbB 2呈阴性，并且似乎与预后不良的基底乳腺癌具有共同的特性。
The estrogen receptor alpha (ER alpha), progesterone receptor (PR), and erbB2 (Her2 in humans) are important prognostic markers of human breast cancer, and they are variably expressed in different subtypes of breast cancer. The basal subtype, for example, is negative for ERa, PR, and Her2 by immunohistochemistry. We investigated the expression of these signaling molecules in enriched populations of mouse mammary stem cells and luminal cells that were isolated according to their differential expression of CD24 and the alpha 6 beta 1-integrin complex. We found that the basal population, which is enriched in mouse mammary stem cells, did not express ERa, PR, or ErbB2/Her2 but did express epidermal growth factor receptor (EGFR)/ ErbB1, whereas the subset of cells enriched for luminal cells expressed ERa (37% of cells) and PR (40% of cells) but not ErbB2/Her2 or EGFR/ErbB1. Ovariectomy confirmed the importance of estrogen signaling to luminal cell proliferation but had no effect on the size of the mouse mammary stem cell-enriched population. Thus, mouse mammary stem cells were negative for ER alpha, PR, and ErbB2 and appeared to share common properties with poor-prognosis basal breast cancer.