A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression
A novel peptide RIFV suppresses human adipocyte differentiation through the inhibition of C/EBP-β expression
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一种新型肽 RIFV 通过抑制 C/EBP-β 表达来抑制人类脂肪细胞分化
DOI：
10.1186/s12986-019-0414-z
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发表时间：
2019-12
期刊：
影响因子：
--
通讯作者：
Chen ling
中科院分区：
文献类型：
--
作者：
Zhang wen;Shendan;Li yun;Zhong hong;Wang xing;Cui xianwei;Shi chunmei;Ji chenbo;Guo xirong;Chen ling
研究背景肥胖是一种全球性的流行病，它增加了代谢综合征的风险。然而，肥胖症的治疗药物仍然稀缺。近年来，肽类被确定为新的生物调节剂。RIFV（R-I-F-V-P-I-K-G-R-P-A-P）是本实验室从肽库中筛选出的一种新的活性肽。采用CCK-8法和流式细胞仪检测RIFV对细胞增殖、凋亡和细胞周期的影响。分别采用qRT-PCR和western blot检测脂肪形成相关基因的mRNA和蛋白水平。通过拯救实验验证RIFV是否能通过靶向C/EBP-β调控脂肪细胞分化。结果RIFV能抑制人白色脂肪细胞的分化，但不影响其增殖。此外，RIFV还可以抑制从腹股沟脂肪组织分离的小鼠原代白色前脂肪细胞的分化。此外，RIFV可能通过抑制成脂基因C/EBP-β.ConclusionsOur结果表明，RIFV可能是一种新的脂肪细胞分化的重要调节剂，并代表了肥胖症和相关并发症的治疗策略，对脂肪形成的抑制作用。
BackgroundObesity is a global epidemic disease that increases the risk of metabolic syndrome. However, therapeutic drugs for obesity are still scarce. In recent years, peptides have been identified as new biological regulators. RIFV (R-I-F-V-P-I-K-G-R-P-A-P), a novel active peptide from our peptide database.MethodsWe performed oil red O staining and triglyceride measurement to analyze the influence of RIFV on white preadipocytes differentiation. Then the effects of RIFV on cell proliferation, apoptosis and cell cycle were determined by using CCK-8 assay and flow cytometry. The mRNA and protein levels of adipogenesis-related genes were respectively detected by qRT-PCR and western blot. Rescue experiment was conducted to confirm whether RIFV could regulate adipocytes differentiation via targeting C/EBP-β. Finally, the luciferase reporter gene assay was performed to verify the regulation of RIFV on C/EBP-β gene.ResultsRIFV was revealed to inhibit the differentiation of human white adipocytes without affecting their proliferation. Additionally, RIFV could also suppress the differentiation of mouse primary white preadipocytes isolated from inguinal fat tissues. Furthermore, RIFV may have an inhibitory effect on adipogenesis by inhibiting the regulation of the adipogenic gene C/EBP-β.ConclusionsOur results indicated that RIFV may be a novel essential regulator of adipocyte differentiation and represents a therapeutic strategy for obesity and related complications.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
3.5
作者：
R. Troke;T. Tan;S. Bloom
通讯作者：
R. Troke;T. Tan;S. Bloom
影响因子：
4.2
作者：
Sethupathy P
通讯作者：
Sethupathy P
影响因子：
8.2
作者：
Sánchez-Garrido MA;Brandt SJ;Clemmensen C;Müller TD;DiMarchi RD;Tschöp MH
通讯作者：
Tschöp MH
影响因子：
5.4
作者：
Kim YM;Kim IH;Choi JW;Lee MK;Nam TJ
通讯作者：
Nam TJ