HEPATITIS-B VIRUS INTEGRATION IN HEPATITIS-B VIRUS RELATED HEPATOCELLULAR-CARCINOMA IN CHILDHOOD
HEPATITIS-B VIRUS INTEGRATION IN HEPATITIS-B VIRUS RELATED HEPATOCELLULAR-CARCINOMA IN CHILDHOOD
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DOI：
10.1016/0270-9139(91)92446-f
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发表时间：
1991-02-01
期刊：
影响因子：
13.5
通讯作者：
CHEN, DS
中科院分区：
文献类型：
--
作者：
CHANG, MH;CHEN, PJ;CHEN, DS
在台湾，肝细胞癌是5至14岁儿童的主要恶性肿瘤之一。我们研究了8例血清HBsAg阳性和母亲HBsAg阳性的儿童肝细胞癌和非肿瘤肝组织中的B型肝炎病毒DNA的状态，其中5例儿童的肝细胞癌组织显示B型肝炎病毒DNA整合到宿主细胞DNA序列中。在四个孩子和一个孩子的多个网站整合模式的单网站整合模式进行了演示。在其余3例儿童中，肿瘤组织中无法显示B型肝炎病毒DNA。以B型肝炎病毒基因组的亚基因组片段为探针，我们发现X基因片段和表面抗原基因片段是最保守的序列。B型肝炎病毒DNA在儿童肝细胞癌中的单位点整合可能击中了关键区域，导致插入突变和肝细胞癌的早期发生。儿童期肝细胞癌潜伏期短，早期接触环境致癌物较少，可能为研究B型肝炎病毒的致癌潜力提供一个良好的模型。
In Taiwan, hepatocellular carcinoma is one of the major malignancies in children between 5 and 14 yr of age. We studied the status of hepatitis B virus DNA in the hepatocellular carcinoma and nontumorous liver tissues of eight children with positive serum HBsAg and maternal HBsAg.The hepatocellular carcinoma tissues from five of the eight children showed integration of hepatitis B virus DNA into host cellular DNA sequences. A pattern of single-site integration in four children and a multiple-site integration pattern in one child were demonstrated. In the remaining three children, hepatitis B virus DNA could not be demonstrated in the tumor tissues. Using subgenomic fragments of the hepatitis B virus genome as probes, we found that the X gene fragment and the surface antigen gene fragment were the most conserved sequences. The single-site integration of hepatitis B virus DNA in childhood hepatocellular carcinoma may have hit the critical region, resulting in insertional mutagenesis and early development of hepatocellular carcinoma. With a short incubation period and less exposure to environmental carcinogens during early life, childhood hepatocellular carcinoma may provide a good model to study the carcinogenic potential of hepatitis B virus.