Immunization with glutathione S-transferase and mutant toxic shock syndrome toxin 1 fusion protein protects against Staphylococcus aureus infection
Immunization with glutathione S-transferase and mutant toxic shock syndrome toxin 1 fusion protein protects against Staphylococcus aureus infection
复制标题
DOI：
10.1016/j.femsim.2005.01.010
复制
发表时间：
2005-07-01
影响因子：
--
通讯作者：
Nakane, A
中科院分区：
文献类型：
--
作者：
Cui, JC;Hu, DL;Nakane, A
为了研究谷胱甘肽S-转移酶（GST）和突变型中毒性休克综合征毒素1（mTSST-1）融合蛋白的免疫是否可以预防金黄色葡萄球菌感染，我们纯化了一种无毒的突变型GST-mTSST-1融合蛋白。用 GST-mTSST-1 加明矾佐剂对小鼠进行免疫，然后用活的金黄色葡萄球菌进行攻击。结果显示，GST-mTSST-1免疫组的存活率高于未免疫组小鼠，且脏器内细菌计数显着低于未免疫组小鼠。用GST-mTSST-1免疫强烈诱导TSST-1特异性抗体的产生，特别是免疫球蛋白G1和免疫球蛋白G2b。此外，GST-mTSST-1免疫小鼠的血清样本还显着抑制TSST-1从小鼠脾细胞产生干扰素-γ和肿瘤坏死因子-α。这些结果表明，接种 GST-mTSST-1 可提供针对金黄色葡萄球菌感染的保护，并且该保护可能是由 TSST-1 中和抗体介导的。 (c) 2005 年欧洲微生物学会联合会。由 Elsevier B.V. 出版。保留所有权利。
To investigate whether immunization with glutathione S-transferase (GST) and mutant toxic shock syndrome toxin 1 (mTSST-1) fusion protein can protect against Staphylococcus aureus infection, we purified a non-toxic mutant GST-mTSST-1 fusion protein. Mice were immunized with the GST-mTSST-1 plus alum adjuvant and then challenged with viable S. aureus. The results showed that the survival rate of GST-mTSST-1-immunized group was higher and the bacteria counts in the organs were significantly lower than those of the non-immunized mice. Immunization with GST-mTSST-1 induced strongly the production of TSST-1 specific antibodies, especially immunoglobulin G1 and immunoglobulin G2b. Furthermore, the serum samples from GST-mTSST-1-immunized mice also significantly inhibited interferon-gamma and tumor necrosis factor-alpha production from murine spleen cells by TSST-1. These results suggest that vaccination with GST-mTSST-1 provides protection against S. aureus infection and that the protection might be mediated by TSST-1-neutralizing antibody. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.