Microarray-based analysis for hepatocellular carcinoma: From gene expression profiling to new challenges
Microarray-based analysis for hepatocellular carcinoma: From gene expression profiling to new challenges
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DOI：
10.3748/wjg.v13.i10.1487
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发表时间：
2007-03-14
影响因子：
4.3
通讯作者：
Aburatani, Hiroyuki
中科院分区：
文献类型：
--
作者：
Midorikawa, Yutaka;Makuuchi, Masatoshi;Aburatani, Hiroyuki
各种基因的突变和表达变化的积累导致了肿瘤的发生，而微阵列技术的发展使我们能够识别在肿瘤发生中全面的基因表达变化。许多研究已经将这一技术应用于肝细胞癌，并发现了一些候选基因，它们可以作为肿瘤分期、复发和预后预测以及治疗选择的生物标志物。其中一些靶分子已被用于开发新的抗肝癌血清诊断标志物和治疗靶点，以造福患者。此前，我们将基因表达谱数据与基于临床病理特征的分类进行了比较，如肝炎病毒感染或肝癌进展。下一代基因表达分析将需要将表达谱与其他类型的生物信息系统地集成在一起，如基因组位置、基因功能和序列信息。我们已经报道了基因表达谱和基因座信息之间的整合，这在检测结构基因组异常方面是有效的，例如染色体获得和丢失，其中我们表明基因表达谱受染色体偏向的影响。此外，还对基于阵列的比较基因组杂交分析和利用基因分型阵列进行的等位基因剂量分析进行了综述，并与传统方法进行了比较。(C)2007年WJG出版社。版权所有。
Accumulation of mutations and alterations in the expression of various genes result in carcinogenesis, and the development of microarray technology has enabled us to identify the comprehensive gene expression alterations in oncogenesis. Many studies have applied this technology for hepatocellular carcinoma (HCC), and identified a number of candidate genes useful as biomarkers in cancer staging, prediction of recurrence and prognosis, and treatment selection. Some of these target molecules have been used to develop new serum diagnostic markers and therapeutic targets against HCC to benefit patients. Previously, we compared gene expression profiling data with classification based on clinicopathological features, such as hepatitis viral infection or liver cancer progression. The next era of gene expression analysis will require systematic integration of expression profiles with other types of biological information, such as genomic locus, gene function, and sequence information. We have reported integration between expression profiles and locus information, which is effective in detecting structural genomic abnormalities, such as chromosomal gains and losses, in which we showed that gene expression profiles are subject to chromosomal bias. Furthermore, array-based comparative genomic hybridization analysis and allelic dosage analysis using genotyping arrays for HCC were also reviewed, with comparison of conventional methods. (c) 2007 The WJG Press. All rights reserved.