Effect of bioparticle size on dispersion and retention in monolithic and perfusive beds.
Effect of bioparticle size on dispersion and retention in monolithic and perfusive beds.
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生物颗粒尺寸对整体床和灌注床中分散和保留的影响。
DOI：
10.1016/j.chroma.2010.09.040
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Lenhoff,AbrahamM
中科院分区：
文献类型：
--
作者：
Trilisky,EgorI;Lenhoff,AbrahamM
单组分脉冲响应研究用于比较小分子、蛋白质和病毒在市售单片和灌注离子交换剂上的保留和转运行为。采用基于矩量法的简单算法对时间失真和柱外效应进行了校正。发现时间畸变与理论塔板数成反比。随着生物颗粒尺寸的增加，保留增加，从非洗脱到非吸附状态的转变，随着离子强度的增加变得更加突然。这两个观察定性地解释了粒子表面的静电吸引能的计算。计算还表明，对于足够大的生物颗粒，如病毒或细胞，流体动力学阻力可以促进洗脱。在非吸附条件下，塔板高度仅随流速微弱增加，且偏斜保持不变。随着保留率的增加，蛋白质的板高显著增加。通过渗透率而不是珠直径缩放板高度，以使不同固定相之间能够进行比较。
Single-component pulse response studies were used to compare the retention and transport behavior of small molecules, proteins, and a virus on commercially available monolithic and perfusive ion-exchangers. Temporal distortion and extra-column effects were corrected for using a simple algorithm based on the method of moments. It was found that temporal distortion is inversely related to the number of theoretical plates. With increasing bioparticle size, retention increased and the transition from a non-eluting to a non-adsorbing state with increasing ionic strength became more abrupt. Both of these observations are qualitatively explained by calculations of particle–surface electrostatic attractive energy. Calculations also suggest that, for sufficiently large bioparticles, such as viruses or cells, hydrodynamic drag can promote elution. Under non-adsorbing conditions, plate height increased only weakly with flow rate and the skew remained unchanged. With increasing retention, plate height increased dramatically for proteins. Plate height was scaled by permeability rather than bead diameter to enable comparison among different stationary phases.