Hybridization between an endangered freshwater fish and an introduced congeneric species and consequent genetic introgression
Hybridization between an endangered freshwater fish and an introduced congeneric species and consequent genetic introgression
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DOI：
10.1371/journal.pone.0212452
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发表时间：
2019-02-14
期刊：
影响因子：
3.7
通讯作者：
Matsuba, Hideki
中科院分区：
文献类型：
--
作者：
Hata, Hiroki;Uemura, Yohsuke;Matsuba, Hideki
生物的人工移植和随之而来的侵入性杂交会导致本地物种的灭绝。在日本的松山，一种本地的刀鱼，Tanakia lanceolata，与另一种刀鱼，T。limbata，最近从日本九州西部引入。在过去的30年里，松山平原上的两种淡水蚌类物种，日本原节蚌和道格拉斯节蚌的鳃中产卵。为了评估入侵杂交的效果，我们测定了T。lanceolata和T. limbata进行形态学分析，推断其生态位重叠。我们收集了Tanakia spp的成年个体。并根据6个微卫星位点和线粒体细胞色素B序列对它们进行基因分型。用几何形态测量学方法分析了它们的体节特征和体型。我们发现，10.9%的所有收集的个人是杂交种。而T. lanceolata在下游分布较密集，T. limbata分布于上游，其杂种分布广泛，覆盖了整个原生T.披针形。体高、臀鳍长、体长、体长、臀鳍长、臀鳍宽、臀鳍长、臀鳍宽limbata比T. lanceolata，但它们的杂种在形态上高度变异，涵盖了双亲的变种，并广泛分布于双亲的栖息地。杂交在两个方向都发生过，但引入了T。limbata雌性和本地T.披针形的雄性更有可能杂交。本研究表明，与引入的T. limbata是T. lanceolata通过遗传渐渗和替代其在溪流中的生态位。
Artificial transplantation of organisms and consequent invasive hybridization can lead to the extinction of native species. In Matsuyama, Japan, a native bitterling fish, Tanakia lanceolata, is known to form hybrids with another bitterling species, T. limbata, which was recently introduced from western Kyushu, Japan. These bitterlings spawn in the gills of two freshwater unionid species, Pronodularia japanensis and Nodularia douglasiae nipponensis, which have rapidly declined on the Matsuyama Plain in the past 30 years. To gauge the effect of invasive hybridization, we determined the genetic introgression between T. lanceolata and T. limbata and analyzed the morphology of these species and their hybrids to infer their niche overlap. We collected adult individuals of Tanakia spp. and genotyped them based on six microsatellite loci and mitochondrial cytochrome b sequences. We analyzed their meristic characters and body shapes by geometric morphometrics. We found that 10.9% of all individuals collected were hybrids. Whereas T. lanceolata were more densely distributed downstream and T. limbata were distributed upstream, their hybrids were widely distributed, covering the entire range of native T. lanceolata. The body height and anal fin length of T. limbata were greater than those of T. lanceolata, but their hybrids were highly morphologically variable, covering both parental morphs, and were widely distributed in the habitats of both parental species. Hybridization has occurred in both directions, but introduced T. limbata females and native T. lanceolata males are more likely to have crossed. This study shows that invasive hybridization with the introduced T. limbata is a potential threat to the native population of T. lanceolata via genetic introgression and replacement of its niche in streams.