Structural and functional analysis of pTB6 from Bifidobacterium longum
Structural and functional analysis of pTB6 from Bifidobacterium longum
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DOI：
10.1271/bbb.69.422
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发表时间：
2005-02-01
影响因子：
1.6
通讯作者：
Kano, Y
中科院分区：
文献类型：
--
作者：
Tanaka, K;Samura, K;Kano, Y
确定了来自长双歧杆菌的 pTB6 (3,624 bp) 的完整核苷酸序列。该质粒与滚环复制 (RCR) 质粒 pKJ36 和 pB44 的核苷酸 (nuc) 序列有 95% 同源性，RepB aa 序列也有 92% 同源性，表明 pTB6 通过滚环机制进行复制。我检测到的推定 MembB、MobA 和 orf (Orf) 编码蛋白对于质粒复制来说不是必需的。我们从 pTB6 和 pUC18 构建了固定穿梭载体，该载体以 2.5 X 10(6) 转化子/杯 DNA 的高效率转化长双歧杆菌。
The complete nucleotide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB aa sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44, suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2.5 X 10(6) transformants/mug DNA.