A novel gene, ecl1+, extends the chronological lifespan in fission yeast
A novel gene, ecl1+, extends the chronological lifespan in fission yeast
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DOI：
10.1111/j.1567-1364.2008.00379.x
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发表时间：
2008-06-01
影响因子：
3.2
通讯作者：
Aiba, Hirofumi
中科院分区：
文献类型：
--
作者：
Ohtsuka, Hokuto;Mita, Satoka;Aiba, Hirofumi
我们已经从裂糖菌中鉴定出一个新的基因，我们将其命名为ecl1(+)（按时间顺序延长寿命的基因）。当在高拷贝数质粒上提供ecl1（+）时，它在进入固定期后延长了Delta sty1 MAP激酶突变体和野生型细胞的活力。Ecl1（+）编码一种80个氨基酸的多肽，目前尚未在数据库中标注。ecl1(+)-mRNA在生长期由对数期转入平稳期时短暂增加。Ecl1蛋白定位于细胞核中。热量限制延长了野生型和δ型ecl1细胞的按时间顺序排列的寿命，但没有延长过量生产ecl1（+）的细胞的寿命。当Ecl1过量产生时，Delta pka1突变体几乎没有表现出生存能力的额外扩展。当Ecl1过量产生时，Pka1负调控的ste11（+）基因被上调。根据这些结果，我们提出Ecl1过量产生的影响可能主要与pka1依赖途径相关并对其产生负面影响。
We have identified a novel gene from Schizosaccharomyces pombe that we have named ecl1(+) (extender of the chronological lifespan). When ecl1(+) is provided on a high-copy number plasmid, it extends the viability of both the Delta sty1 MAP kinase mutant and the wild-type cells after entry into the stationary phase. ecl1(+) encodes an 80-amino acid polypeptide that had not been annotated in the current database. The ecl1(+)-mRNA increases transiently when the growth phase is changed from the log phase to the stationary phase. The Ecl1 protein is localized in the nucleus. Calorie restriction extends the chronological lifespan of wild-type and Delta ecl1 cells but not ecl1(+)-overproducing cells. The Delta pka1 mutant shows little, if any, additional extension of viability when Ecl1 is overproduced. The ste11(+) gene that is negatively controlled by Pka1 is up regulated when Ecl1 is overproduced. From these results we propose that the effect of Ecl1 overproduction may be mainly linked to and negatively affects the Pka1-dependent pathway.