Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins
Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins
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DOI：
10.1006/geno.2000.6244
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发表时间：
2000-08-01
期刊：
影响因子：
4.4
通讯作者：
Liebhaber, SA
中科院分区：
文献类型：
--
作者：
Makeyev, AV;Liebhaber, SA
我们已经鉴定了两个新的人类基因，其编码的蛋白质与两个先前表征的RNA结合蛋白α CP-1和α CP-2具有高度的序列同一性。这两个新基因α CP-3和α CP-4被预测编码具有三重KH结构域的蛋白质。KH结构域的数量和组织、它们的序列以及相邻区域的序列在所有四种α CP蛋白中是保守的。这些蛋白质的共同进化起源由相应基因中外显子-内含子结构的保守性所证实。α CP-1和α CP-2（先前报道）的图谱位置以及α CP-3和α CP-4（本报告）的图谱位置表明，4个α CP基因座分散在人类基因组中; α CP-3和α CP-4分别定位于21q22.3和3 p21，相应的小鼠直向同源物分别定位于小鼠基因组的同线区域10 B5和9 F1 F2。人类基因组中的另外两个位点被鉴定为α CP-2加工假基因（PCBP 2 P1，21q22.3和PCBP 2 P2，8 q21-q22）。尽管α CP-3和α CP-4 mRNA的总体水平显著低于α CP-1和α CP-2的水平，但在所有检测的小鼠组织中均发现了α CP-3和α CP-4的转录物。这些数据建立了一个新的亚家族的基因预测编码密切相关的KH含RNA结合蛋白的转录后控制的潜在功能。(C)北京大学出版社.
We have identified two novel human genes encoding proteins with a high level of sequence identity to two previously characterized RNA-binding proteins, alpha CP-1 and alpha CP-2, Both of these novel genes, alpha CP-3 and alpha CP-4, are predicted to encode proteins with triplicated KH domains. The number and organization of the KH domains, their sequences, and the sequences of the contiguous regions are conserved among all four alpha CP proteins. The common evolutionary origin of these proteins Is substantiated by conservation of exon-intron organization in the corresponding genes. The map positions of alpha CP-1 and alpha CP-2 (previously reported) and those of alpha CP-3 and alpha CP-4 (present report) reveal that the four alpha CP loci are dispersed in the human genome; alpha CP-3 and alpha CP-4 mapped to 21q22.3 and 3p21, and the respective mouse orthologues mapped to syntenic regions of the mouse genome, 10B5 and 9F1F2, respectively. Two additional loci in the human genome were identified as alpha CP-2 processed pseudogenes (PCBP2P1, 21q22.3, and PCBP2P2, 8q21-q22). Although the overall levels of alpha CP-3 and alpha CP-4 mRNAs are substantially lower than those of alpha CP-1 and alpha CP-2, transcripts of alpha CP-3 and alpha CP-4 were found in all mouse tissues tested. These data establish a new subfamily of genes predicted to encode closely related KH-containing RNA-binding proteins with potential functions in posttranscriptional controls. (C) 2000 Academic Press.