Quantitative structural changes in white and gray matter 1 year following traumatic brain injury in rats
Quantitative structural changes in white and gray matter 1 year following traumatic brain injury in rats
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DOI：
10.1007/s00401-001-0510-8
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发表时间：
2002-06-01
影响因子：
12.7
通讯作者：
Dietrich, WD
中科院分区：
文献类型：
--
作者：
Bramlett, HM;Dietrich, WD
有证据表明，人类头部创伤后会出现慢性萎缩，这可能与长期功能缺陷有关。然而，使用已建立的创伤性脑损伤（TBI）模型，只有有限的数据可用于阐明进行性灰质和白色萎缩的程度。在本研究中，雄性Sprague-Dawley大鼠接受中度（2.01-2.21 atm）旁矢状流体冲击脑损伤（n=7）或假手术（n=3），并在TBI后1年处死。通过轴突获得半透明切片，并用苏木精和伊红双重染色以区分灰质结构，并用Luxol坚牢蓝染色以显示白色物质。获得以下结构的同侧和对侧体积测量值：大脑皮质、海马、齿状回、丘脑、侧脑室、外囊、内囊、大脑脚和胼胝体。TBI大鼠同侧灰质结构的定量评估显示，与假手术动物相比，创伤中心后部的大脑皮质面积测量值显着减少。重要的是，几个白色物质束表现出显着萎缩。TBI组和假手术组的比较显示出显著的（P
There is evidence for chronic atrophy after human head trauma, which may be associated with long-term functional deficits. However, using established models of traumatic brain injury (TBI) only limited data are available for clarifying the extent of progressive gray and white matter atrophy. In the present study, male Sprague-Dawley rats underwent moderate (2.01-2.21 atm) para-sagittal fluid percussion brain injury (n=7) or sham (n=3) surgery and were killed at I year post TBI. Semiserial sections were obtained through the neuraxis and double stained with hematoxylin and eosin to demarcate gray matter structures and Luxol fast blue for white matter visualization. Both ipsilateral and contralateral volume measurements were obtained for the following structures: cerebral cortex, hippocampus, dentate gyrus, thalamus, lateral ventricle, external capsule, internal capsule, cerebral peduncle and corpus callosum. Quantitative assessment of ipsilateral gray matter structures from TBI rats revealed significant reductions in cerebral cortical area measurements posterior from the trauma epicenter compared to sham animals. Importantly, several white matter tracts exhibited dramatic atrophy. A comparison of TBI and sham groups demonstrated a significant (P