Mouse Model of Middle Cerebral Artery Occlusion
Mouse Model of Middle Cerebral Artery Occlusion
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DOI：
10.3791/2761
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发表时间：
2011-02-01
影响因子：
1.2
通讯作者：
Chou, Wen-Hai
中科院分区：
文献类型：
--
作者：
Chiang, Terrance;Messing, Robert O.;Chou, Wen-Hai
中风是美国最常见的致命性神经系统疾病（1）。大多数中风（88%）是由于大脑血管阻塞（缺血性中风）（2）。由于大多数缺血性卒中（约80%）发生在大脑中动脉（MCA）区域（3），因此已开发的许多动物卒中模型都集中在该动脉。大脑中动脉闭塞（MCAO）的管腔内单股模型涉及将外科细丝插入颈外动脉并将其向前穿入颈内动脉（伊卡），直至尖端闭塞MCA的起源，导致血流停止和随后的MCA区域脑梗死（4）。该技术可用于模拟永久性或暂时性闭塞（5）。如果在一定间隔（30分钟、1小时或2小时）后拆除缝线，则实现再灌注（短暂性MCAO）;如果细丝留在原位（24小时），则该手术适合作为永久性MCAO的模型。这种技术不需要颅骨切除术，这是一种神经外科手术，可以切除一部分颅骨，这可能会影响颅内压和温度（6）。它已成为模拟大鼠和小鼠永久性和短暂性局灶性脑缺血的最常用方法（7，8）。为了评估脑梗死的程度，我们用氯化（2，3，5）-三苯基四氮唑（TTC）对脑切片进行染色，以识别缺血性脑组织（9）。在本视频中，我们演示了MCAO方法和TTC染色法测定梗死面积。
Stroke is the most common fatal neurological disease in the United States (1). The majority of strokes (88%) result from blockage of blood vessels in the brain (ischemic stroke) (2). Since most ischemic strokes (similar to 80%) occur in the territory of middle cerebral artery (MCA) (3), many animal stroke models that have been developed have focused on this artery. The intraluminal monofilament model of middle cerebral artery occlusion (MCAO) involves the insertion of a surgical filament into the external carotid artery and threading it forward into the internal carotid artery (ICA) until the tip occludes the origin of the MCA, resulting in a cessation of blood flow and subsequent brain infarction in the MCA territory (4). The technique can be used to model permanent or transient occlusion (5). If the suture is removed after a certain interval (30 min, 1 h, or 2 h), reperfusion is achieved (transient MCAO); if the filament is left in place (24 h) the procedure is suitable as a model of permanent MCAO. This technique does not require craniectomy, a neurosurgical procedure to remove a portion of skull, which may affect intracranial pressure and temperature (6). It has become the most frequently used method to mimic permanent and transient focal cerebral ischemia in rats and mice (7,8). To evaluate the extent of cerebral infarction, we stain brain slices with (2,3,5)-triphenyltetrazolium chloride (TTC) to identify ischemic brain tissue (9). In this video, we demonstrate the MCAO method and the determination of infarct size by TTC staining.