DISTRIBUTION OF COLLAGENS TYPE-I, TYPE-III AND TYPE-V IN THE PANCREAS OF RAT, DOG, PIG AND MAN
DISTRIBUTION OF COLLAGENS TYPE-I, TYPE-III AND TYPE-V IN THE PANCREAS OF RAT, DOG, PIG AND MAN
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DOI：
10.1007/bf00303087
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发表时间：
1994-07-01
影响因子：
3.6
通讯作者：
VANSCHILFGAARDE, R
中科院分区：
文献类型：
--
作者：
VANDEIJNEN, JHM;VANSUYLICHEM, PTR;VANSCHILFGAARDE, R
在大鼠、猪、狗和人的胰腺组织中，用免疫化学方法测定了I型、III型和V型胶原的存在。在岛周区域和小叶、小叶和腺泡隔中，对I型胶原的反应较弱（猪、狗）或中等（大鼠、人），而对III型胶原的反应则很发达。在大鼠和犬中，后一种反应存款在叶和腺泡隔上是突出的。这些元素在猪和人中仅显示出中等反应强度。岛周区域显示出对III型胶原蛋白的弱（大鼠、犬、人）或非常弱（猪）反应。抗V型胶原蛋白在肺叶和小叶间隔中发生中度反应（大鼠、狗、人）或较弱反应（猪）。腺泡隔显示中度（大鼠、犬、人）或极弱（猪）反应。关于胰腺细胞外基质中胶原化合物的类型和分布的这一信息可能导致分化的酶促胰腺解离，并最终增加胰岛产量和改善用于移植目的的胰岛分离程序的再现性。
The presence of collagens type I, type III and type V was determined immunohistochemically in pancreatic tissue of rat, pig, dog and man. The reaction to anti-collagen type I is weak (pig, dog) or moderate (rat, man) in the peri-insular region and in the lobar, lobular and acinar septa, whereas the reaction to anti-collagen type III is well developed. In rat and dog, the latter reaction deposit on the lobar and acinar septa is prominent. These elements only show a moderate reaction intensity in pig and man. The peri-insular region displays a weak (rat, dog, man) or very weak (pig) reaction against collagen type III. Anti-collagen type V reacts moderately (rat, dog, man) or weakly (pig) in the lobar and lobular septa. The acinar septa show a moderate (rat, dog, man) or very weak (pig) reaction. This information regarding the types and distribution of the collagenous compounds in pancreatic extracellular matrix could lead to differentiated enzymatic pancreas dissociation and, ultimately, increased islet yield and improved reproducibility of pancreatic islet isolation procedures for transplantation purposes.