Semi-automated, Membrane-Based Protocol for DNA Isolation from Plants
Semi-automated, Membrane-Based Protocol for DNA Isolation from Plants
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DOI：
10.1007/s11105-008-0029-4
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发表时间：
2008-09-01
影响因子：
2.1
通讯作者：
Hebert, Paul D. N.
中科院分区：
文献类型：
--
作者：
Ivanova, Natalia V.;Fazekas, Aron J.;Hebert, Paul D. N.
许多植物因其高浓度的多糖、多酚等次生代谢产物而被认为是DNA提取的难点。已经开发了几种协议来克服这个问题，但它们通常很耗时，无法扩展以实现高吞吐量，并且与自动化不兼容。虽然有多种商业试剂盒可用于植物DNA分离，但它们的成本很高，并且这些试剂盒通常具有有限的分类适用性。在以前的研究中，我们开发了一种廉价的自动化友好协议，用于从动物组织中提取DNA。在这里，我们证明了一个类似的协议允许从植物中分离DNA。
Many plant species are considered difficult for DNA isolation due to their high concentrations of secondary metabolites such as polysaccharides and polyphenols. Several protocols have been developed to overcome this problem, but they are typically time-consuming, not scalable for high throughput and not compatible with automation. Although a variety of commercial kits are available for plant DNA isolation, their cost is high and these kits usually have limited taxonomic applicability. In a previous study we developed an inexpensive automation-friendly protocol for DNA extraction from animal tissues. Here we demonstrate that a similar protocol allows DNA isolation from plants.