Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals.
Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals.
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DOI：
10.1038/s41467-021-22920-8
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发表时间：
2021-05-07
影响因子：
16.6
通讯作者：
Osbourn A
中科院分区：
文献类型：
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作者：
Li Y;Leveau A;Zhao Q;Feng Q;Lu H;Miao J;Xue Z;Martin AC;Wegel E;Wang J;Orme A;Rey MD;Karafiátová M;Vrána J;Steuernagel B;Joynson R;Owen C;Reed J;Louveau T;Stephenson MJ;Zhang L;Huang X;Huang T;Fan D;Zhou C;Tian Q;Li W;Lu Y;Chen J;Zhao Y;Lu Y;Zhu C;Liu Z;Polturak G;Casson R;Hill L;Moore G;Melton R;Hall N;Wulff BBH;Doležel J;Langdon T;Han B;Osbourn A
真核生物的非随机基因组织在基因组进化中起着重要作用。在这里，我们研究了燕麦中用于生产防御化合物的生物合成基因簇——阿维那酸簇的起源。我们阐明了这12个基因簇的结构和组织，表征了最后两个缺失的途径步骤，并通过瞬时表达重建了烟草中的整个途径。我们发现，由于燕麦基因组的亚端粒区与其他禾本科植物缺乏同源性，该基因序列与生物合成途径近似共线性，因此该集群是在燕麦基因组的亚端粒区分化后重新形成的。我们推测，晚期通路基因离端粒最远的位置可能会减轻“自我中毒”的情况，在这种情况下，由于端粒基因缺失，有毒中间体会积累。我们的研究揭示了一个显著的适应性进化的例子，该进化是由显著的基因组可塑性支撑的。阿维纳酸生物合成基因簇的基因组组织和起源仍然未知。在此，作者组装了二倍体燕麦荞麦的基因组，揭示了连续基因的结构和组织，表征了最后两个缺失的途径步骤，并探讨了该途径在谷物中的起源。
Non-random gene organization in eukaryotes plays a significant role in genome evolution. Here, we investigate the origin of a biosynthetic gene cluster for production of defence compounds in oat—the avenacin cluster. We elucidate the structure and organisation of this 12-gene cluster, characterise the last two missing pathway steps, and reconstitute the entire pathway in tobacco by transient expression. We show that the cluster has formed de novo since the divergence of oats in a subtelomeric region of the genome that lacks homology with other grasses, and that gene order is approximately colinear with the biosynthetic pathway. We speculate that the positioning of the late pathway genes furthest away from the telomere may mitigate against a ‘self-poisoning’ scenario in which toxic intermediates accumulate as a result of telomeric gene deletions. Our investigations reveal a striking example of adaptive evolution underpinned by remarkable genome plasticity. The genomic organization and origin of the avenacin biosynthetic gene cluster remain unknown. Here, the authors assemble the genome of diploid oat Avena strigosa, reveal the structure and organization of the consecutive genes, characterize the last two missing pathway steps, and investigate the origin of the pathway in cereals.