BACTERIA AND AETIOLOGY OF CANCER OF LARGE BOWEL
BACTERIA AND AETIOLOGY OF CANCER OF LARGE BOWEL
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DOI：
10.1136/gut.10.5.334
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发表时间：
1969-01-01
期刊：
影响因子：
24.5
通讯作者：
WILLIAMS, RE
中科院分区：
文献类型：
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作者：
ARIES, V;CROWTHER, JS;WILLIAMS, RE
材料和方法：对48名居住在坎帕拉和附近的乌干达健康成年人和40名居住在伦敦的英国健康成年人的新鲜排泄物进行了检查。将样本保存为含10%甘油的肉浸液肉汤中的10%混悬液，在固体二氧化碳上冷冻（Drasar、Shiner和McLeod，1969），以便运输和储存;已发现细菌在这些条件下存活良好。通过先前描述的方法（Drasar，1967）进行微小修改培养标本。为了补偿培养基、孵育温度和操作技术的微小波动，在每个检测日检查大约相同数量的英国和乌干达标本。研究胆盐降解的方法见其他文献（Hill和Drasar，1968; Aries、Crowther、Drasar和Hill，1969）。
MATERIALS AND METHODSSamples of freshly voided faeces from 48 healthy Ugandan adults living in and arotmd Kampala and from 40 healthy English adults living in London were examined. Specimens were preserved for transport and storage as a 10% suspension in meat infusion broth containing 10% glycerol frozen on solid carbon dioxide (Drasar, Shiner, and McLeod, 1969); the bacteria have been found to survive well under these conditions. Specimens were cultivated by the methods described previously (Drasar, 1967) with minor modifications. Approximately equal numbers of English and Ugandan specimens were examined on each day of testing in order to compensate for minor fluctuations in culture media, incubation tenperatures, and operational techniques. Methods for investigating the degradation of bile salts are described elsewhere (Hill and Drasar, 1968; Aries, Crowther, Drasar, and Hill, 1969).