Preparation of PEG and protein co-immobilized upconversion nanophosphors as near-infrared biolabeling materials
Preparation of PEG and protein co-immobilized upconversion nanophosphors as near-infrared biolabeling materials
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DOI：
10.2494/photopolymer.21.183
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发表时间：
2008-06
影响因子：
0.8
通讯作者：
Masao Kamimura;D. Miyamoto;Yu Saito;K. Soga;Y. Nagasaki
中科院分区：
文献类型：
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作者：
Masao Kamimura;D. Miyamoto;Yu Saito;K. Soga;Y. Nagasaki
上转换荧光粉有望用作荧光生物标记材料。采用均匀沉淀法合成了一种上转换发光材料-掺铒Y2 O3（Y2 O3：Er）纳米颗粒（YNPs）。将聚乙二醇-b-聚丙烯酸（PEG-b-PAAc）固定在YNP表面，以提高分散稳定性和防止非特异性吸附。为了将蛋白质固定在YNP表面，进行了PEG-b-PAAc和牛血清白蛋白（BSA）作为模型蛋白的共固定。抗BSA抗体平板法证实了PEG/BSA共固定YNPs（PEG/BSA-YNPs）的特异性分子识别。所得配合物是一种很有前途的高性能近红外生物标记材料。
Upconversion phosphors are expected to be used as fluorescence biolabeling materials. One of upconversion phosphors, erbium ion-doped-Y2O3 (Y2O3:Er) nanoparticles (YNPs) was synthesized by homogeneous precipitation method. Poly(ethylene glycol)-b-Poly(acrylic acid) (PEG-b-PAAc) was immobilized on the surface of YNP to improve dispersing stability and prevention of nonspecific adsorption. In order to install protein on the YNP surface, co-immobilization of PEG-b-PAAc and bovine serum albumin (BSA) as a model protein was carried out. The specific molecular recognition of PEG/BSA co-immobilized YNPs (PEG/BSA-YNPs) was confirmed by anti-BSA antibody plate assay. The obtained complex is a promising near-infrared biolabeling materials with high performances.