Activation of silenced cytokine gene promoters by the synergistic effect of TBP-TALE and VP64-TALE activators.
Activation of silenced cytokine gene promoters by the synergistic effect of TBP-TALE and VP64-TALE activators.
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DOI：
10.1371/journal.pone.0095790
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Anthony K;More A;Zhang X
Recent work has shown that the combinatorial use of multiple TALE activators can selectively activate certain cellular genes in inaccessible chromatin regions. In this study, we aimed to interrogate the activation potential of TALEs upon transcriptionally silenced immune genes in the context of non-immune cells. We designed a unique strategy, in which a single TALE fused to the TATA-box binding protein (TBP-TALE) is coupled with multiple VP64-TALE activators. We found that our strategy is significantly more potent than multiple TALE activators alone in activating expression of IL-2 and GM-CSF in diverse cell origins in which both genes are otherwise completely silenced. Chromatin analysis revealed that the gene activation was due in part to displacement of a distinctly positioned nucleosome. These studies provide a novel epigenetic mechanism for artificial gene induction and have important implications for targeted cancer immunotherapy, DNA vaccine development, as well as rational design of TALE activators.
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DOI：
10.4049/jimmunol.1003173
发表时间：
2011-09-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ishihara S;Schwartz RH
通讯作者：
Schwartz RH
影响因子：
2.3
作者：
Fitzgerald, SM;Chi, DS;Krishnaswamy, G
通讯作者：
Krishnaswamy, G
影响因子：
4.4
作者：
Attema, JL;Reeves, R;Shannon, AF
通讯作者：
Shannon, AF
影响因子：
3.7
作者：
Geissler R;Scholze H;Hahn S;Streubel J;Bonas U;Behrens SE;Boch J
通讯作者：
Boch J
影响因子：
4
作者：
Hau, Heidi H.;Walsh, Richard J.;Bohjanen, Paul R.
通讯作者：
Bohjanen, Paul R.