Organization of the CYP1A cluster on human chromosome 15:: implications for gene regulation
Organization of the CYP1A cluster on human chromosome 15:: implications for gene regulation
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DOI：
10.1097/00008571-200102000-00001
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发表时间：
2001-02-01
期刊：
影响因子：
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通讯作者：
Gonzalez, FJ
中科院分区：
文献类型：
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作者：
Corchero, J;Pimprale, S;Gonzalez, FJ
测定了人类15号染色体上的CYP1A簇的序列和组织。从BAC文库中分离并测序了一个同时含有CYP1A1和CYP1A2基因的人类基因组克隆。用人基因组DNA进行Southern印迹分析的结果与BAC克隆的结构一致。CYP1A1和CYP1A2基因被一段23kb的片段隔开，该片段不含其他开放阅读框，CYP1A1和CYP1A2基因位于相反的方向，表明这两个基因的5‘侧翼区是共同的。对获得的序列的分析表明，先前报道的CYP1A1和CYP1A2存在异源反应元件(XRE)，以及其他几个可能的XRE序列，这两个基因上游所有XRE的存在表明，一些已知的控制CYP1A1基因表达的调控元件也可以控制CYP1A2基因的表达。《药物遗传学》11：1-6(C)2001，Lippincott Williams&Wilkins。
The sequence and organization of the CYP1A cluster on human chromosome 15 was determined. A human genomic clone from a BAC library, containing both CYP1A1 and CYP1A2 genes, was isolated and sequenced. The results of Southern blot analysis using human genomic DNA were compatible with the structure of the BAC clone. The CYP1A1 and CYP1A2 genes are separated by a 23 kb segment that contains no other open reading frames, The CYP1A1 and CYP1A2 genes are in opposite orientation, revealing that the 5' flanking region is in common between the two genes. Analysis of the sequence obtained revealed the presence of xenobiotic response elements (XREs) previously reported for CYP1A1 and CYP1A2 and several additional consensus sequences for putative XREs, The presence of all the XREs upstream of both genes suggest that some of the regulatory elements known to control CYP1A1 gene expression, could also control CYP1A2 gene expression. Pharmacogenetics 11:1-6 (C) 2001 Lippincott Williams & Wilkins.