Moderate amplifications of the c-myc gene correlate with molecular and clinicopathological parameters in colorectal cancer.
Moderate amplifications of the c-myc gene correlate with molecular and clinicopathological parameters in colorectal cancer.
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DOI：
10.1038/bjc.1998.390
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发表时间：
1998-06
影响因子：
8.8
通讯作者：
Peinado, M A
中科院分区：
文献类型：
--
作者：
Masramon, L;Arribas, R;Tortola, S;Perucho, M;Peinado, M A
C-myc 基因激活是多种类型肿瘤中的常见事件，并且与与癌细胞恶性转化相关的不同细胞过程相关。通过基于任意引物 PCR 的创新 DNA 指纹分析方法，对结直肠癌中的 C-myc 基因扩增进行了分析。该方法需要少量 DNA，使用多个内部对照，并且显得灵敏且可重复。已对 70 例结直肠癌的一系列临床病理学和分子相关性进行了研究。 c-myc 扩增的发生率为 26%，拷贝数增加了两倍到五倍。 C-myc 扩增发生在肿瘤侵袭的较晚期阶段更为频繁 (P < 0.001)，并且与 p53 肿瘤抑制基因的突变相关 (P = 0.048)。 c-myc 扩增的存在表明无病生存期较短，但由于其与 Dukes 分期密切相关，其预后价值值得怀疑。
C-myc gene activation is a common event in multiple types of neoplasia and has been associated with different cellular processes relevant to the malignant transformation of cancer cells. C-myc gene amplification has been analysed in colorectal carcinomas by means of an innovative DNA fingerprinting method based on the arbitrarily primed PCR. This method requires a low amount of DNA, uses multiple internal controls and appears sensitive and reproducible. Clinicopathological and molecular correlates have been investigated in a series of 70 colorectal carcinomas. The incidence of c-myc amplification was 26%, ranging from two- to fivefold increase in copy number. C-myc amplification occurrence was more frequent in more advanced stages of tumour invasion (P < 0.001) and was associated with mutations in the p53 tumour-suppressor gene (P = 0.048). The presence of c-myc amplification was indicative of a shorter disease-free survival period but, because of its strong association with Dukes' stage, its prognostic value is questionable.