Microchromatography of hemoglobins. II. A simplified procedure for the determination of hemoglobin A2.

Microchromatography of hemoglobins. II. A simplified procedure for the determination of hemoglobin A2.
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血红蛋白的微量色谱法。

DOI:
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发表时间:
1975
期刊:
Journal of Laboratory and Clinical Medicine
影响因子:
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通讯作者:
J. Jakway
J. Jakway
中科院分区:
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文献类型:
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作者:
T. H. Huisman;W. A. Schroeder;Brodie An;Mayson Sm;J. Jakway

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利用 DEAE-纤维素(参考文献 1)对 Hb-A2 的微量色谱程序进行了修改,可以在不受样品中任何 Hb-S 干扰的情况下对 Hb-A2 进行定量。使用甘氨酸-KCN 显色剂洗脱 Hb-A2 对显色剂 pH 值的微小变化不太敏感,但很大程度上取决于离子交换剂的 pH 值。
A modification of the microchromatographic procedure for Hb-A2 which utilizes DEAE-cellulose (Reference 1) allows the quantitation of Hb-A2 without interference from any Hb-S in the sample. Elution of Hb-A2 with a glycine-KCN developer is much less sensitive to minor change in pH of the developer, but is greatly dependent on the pH of the ion exchanger.