An improved method for the artificial insemination of mice — oviduct transfer of spermatozoa
An improved method for the artificial insemination of mice — oviduct transfer of spermatozoa
复制标题
小鼠人工授精的改进方法——精子输卵管移植
DOI:
10.1016/s0168-9525(96)90052-9
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发表时间:
1996
影响因子:
11.4
通讯作者:
Rashmi Kothary
中科院分区:
文献类型:
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作者:
Y. Repentigny;Rashmi Kotbary;Rashmi Kothary
A method for the artificial insemination of mice is described, in which a low number of spermatozoa are transferred directly into the ampulla of a pseudopregnant ♀. A similar technique, known as gameto-intrafallopian transfer, has been used in humans. Using freshly collected sperm, 15 of 22 (68%) mice were successfully inseminated and the number of viable embryos recovered varied from 1 to 9. Using frozen or thawed spermatozoa, only 4 of 18 (22%) mice were successfully inseminated.
DOI:
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影响因子:
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