An improved method for the artificial insemination of mice — oviduct transfer of spermatozoa

An improved method for the artificial insemination of mice — oviduct transfer of spermatozoa
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小鼠人工授精的改进方法——精子输卵管移植

DOI:
10.1016/s0168-9525(96)90052-9
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发表时间:
1996
期刊:
影响因子:
11.4
通讯作者:
Rashmi Kothary
Rashmi Kothary
中科院分区:
生物学1区
文献类型:
--
作者:
Y. Repentigny;Rashmi Kotbary;Rashmi Kothary

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描述了一种小鼠人工授精的方法,其中将少量精子直接转移到假孕小鼠的壶腹中。一种类似的技术,称为配子输卵管内移植,已用于人类。使用新鲜收集的精子,22只小鼠中有15只(68%)成功授精,回收的活胚胎数从1到9不等。使用冷冻或解冻的精子,只有4/18(22%)的小鼠成功授精。
A method for the artificial insemination of mice is described, in which a low number of spermatozoa are transferred directly into the ampulla of a pseudopregnant ♀. A similar technique, known as gameto-intrafallopian transfer, has been used in humans. Using freshly collected sperm, 15 of 22 (68%) mice were successfully inseminated and the number of viable embryos recovered varied from 1 to 9. Using frozen or thawed spermatozoa, only 4 of 18 (22%) mice were successfully inseminated.
《1985年度研究成果报告》(1986年)
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