Performance and microbial communities of completely autotrophic denitrification in a bioelectrochemically-assisted constructed wetland system for nitrate removal.
Performance and microbial communities of completely autotrophic denitrification in a bioelectrochemically-assisted constructed wetland system for nitrate removal.
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DOI：
10.1016/j.biortech.2016.12.065
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发表时间：
2017-03
影响因子：
11.4
通讯作者：
Dan Xu;Enrong Xiao;Peng Xu;Yin Zhou;F. He;Qiaohong Zhou;Dong-ying Xu;Zhenbin Wu
中科院分区：
文献类型：
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作者：
Dan Xu;Enrong Xiao;Peng Xu;Yin Zhou;F. He;Qiaohong Zhou;Dong-ying Xu;Zhenbin Wu
在无有机碳源条件下，采用生物电化学辅助人工湿地（BECW）系统处理硝酸盐污染废水。比较了BECW在闭路模式（BECW-C）和在开路模式（BECW-O）的反硝化性能和微生物群落组成。当外加电流为15 mA时，BECW-C的反硝化效率最高，为78.92 ± 3.12%。在BECW-C系统的自养反硝化过程中，未观察到亚硝酸盐的积累。与BECW-O相比，BECW-C的显着更高的反硝化效率表明由于原位产生氢气而增强了反硝化作用。采用16 S rRNA焦磷酸测序技术对BECW-C（10 mA和15 mA）和BECW-O的阳极、阴极和根际土壤细菌群落进行了分析，结果表明，不同处理的土壤微生物群落结构存在差异。研究结果还表明，BECW-C系统中的自养反硝化可能是由嗜硫盐菌和狭义梭菌共同完成的。
A bioelectrochemically-assisted constructed wetland (BECW) system was used to treat nitrate-contaminated wastewater without organic carbon source. The denitrification performance and microbial community composition of a BECW in closed-circuit mode (BECW-C) was compared to a BECW in open-circuit mode (BECW-O). The highest denitrification efficiency (78.92 ± 3.12%) was obtained in the BECW-C with an applied current of 15 mA. No nitrite accumulation was observed during the autotrophic denitrification process in the BECW-C. The significantly higher denitrification efficiency of the BECW-C compared to the BECW-O suggested enhanced denitrification due to in situ generation of hydrogen. The bacterial communities in the anode, cathode and rhizosphere regions collected from the BECW-C (with 10 or 15 mA) and the BECW-O were characterized using 16S rRNA pyrosequencing technology, which revealed different microbial community structures among the treatments. The results also indicated thatThiohalophilusand Clostridiumsensu strictomight be responsible for autotrophic denitrification in the BECW-C.