Survival of exotic Newcastle disease virus in commercial poultry environment following removal of infected chickens
Survival of exotic Newcastle disease virus in commercial poultry environment following removal of infected chickens
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DOI：
10.1637/7161-020104r
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发表时间：
2004-09-01
期刊：
影响因子：
1.4
通讯作者：
McFarland, M
中科院分区：
文献类型：
--
作者：
Kinde, H;Utterback, W;McFarland, M
在2003年的头几个星期，在南加州的商业鸡群中确认了外来新城疫（END）之后，很明显，文献中的病毒存活信息差异很大，很难根据当前的当地情况进行推断。END工作队利用文献中提供的信息和研究科学家的建议，制定了安全处理来自受感染和人口减少场所的粪便的方案。为了在暴发过程中获得更多适用于污染家禽粪便管理的知识，设计并实施了本病毒生存研究。在立即清除受感染的鸡群后，从两个早期感染的商业牧场（由几个房屋组成）对环境拖拭子进行了END病毒检测。对3个猪舍（牧场1）的168个粪便拖拭池、72个粪板拭池、38个堆肥拭池和牧场2的180个粪便带刮拭池共293份样本进行了种群消失后连续21天的ND病毒分离和鉴定分析。从牧场1收集的13个粪便拖拭子池（来自1号和3号房）和2个粪便滴板拭子池（来自3号房）在种群消失后0、1、2、3、4、7、10、12和16天呈END病毒阳性。在种群减少后的第16天，没有从任何样本中分离出END病毒。在整个观察期，2号牧场的所有样本均为阴性。
During the first weeks of 2003, after exotic Newcastle disease (END) was confirmed in commercial layer flocks in Southern California, it became apparent that the virus survival information in the literature varied widely and was difficult to extrapolate to current local conditions. The END Task Force used the information available in the literature and the recommendations of research scientists to establish protocols for safely handling manure from infected and depopulated premises. In an attempt to gain more applicable knowledge in the management of contaminated poultry manure in the Course of the END outbreak, this virus survival study was designed and implemented. Environmental drag swabs were tested for END virus from two of the early-infected commercial ranches that consisted of several houses following immediate removal of the infected flocks. A total of 293 samples, composed of 168 manure drag swab pools, 72 dropping board swab pools, and 38 compost swab pools from 3 houses (ranch 1), and 180 manure belt scraper swab pools from ranch 2 were analyzed for ND virus isolation and characterization for 21 consecurive days postdepopulation. Thirteen manure drag swab pools (from houses 1 and 3) and two manure dropping board swab pools (from house 3) collected from ranch I were positive for END virus at 0, 1, 2, 3, 4, 7, 10, 12, and 16 days postdepopulation. No END virus was isolated after the 16th day following depopulation from any of the samples. All samples from ranch 2 were negative during the entire observation period.