Treatment with antibiotics reduces plasma equol concentration in cynomolgus monkeys (Macaca fascicularis)
Treatment with antibiotics reduces plasma equol concentration in cynomolgus monkeys (Macaca fascicularis)
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DOI：
10.1093/jn/133.7.2262
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发表时间：
2003-07-01
影响因子：
4.2
通讯作者：
Clarkson, TB
中科院分区：
文献类型：
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作者：
Blair, RM;Appt, SE;Clarkson, TB
为了在未来的研究中探索木醇对健康结果的重要性，有必要开发一种方法来减少木马的生产。对照组(n=4)、强力霉素(2.5 mg/kg；n=4)、甲硝唑(125 mg/d；n=3)、卡那霉素(1000 mg/d；n=4)、万古霉素(100 mg/d；n=3)或卡那霉素+万古霉素(n=4)。分别于基础喂养后4h、治疗后4wk和治疗结束后8wk采集血浆样品，分析其异黄酮类化合物的浓度。在基线和抗生素治疗结束时采集粪便拭子，分析革兰氏(+)和革兰氏(-)细菌的生长情况。甲硝唑(955+/-164vs.193+/-53nmol/L)、卡那霉素(545+/-211vs.37.1+/-17.6nmol/L)、万古霉素(607+/-163vs.8.9+/-8.2nmol/L)和卡那霉素+万古霉素(721+/-169vs.17.3+/-17.3nmol/L)治疗后，马儿浓度较治疗前分别下降了80、93、98和99%。多西环素(336+/-87比576+/-76nmol/L)、卡那霉素(168+/-67比374+/-15nmol/L)和卡那霉素+万古霉素(166+/-35比384+/-78nmol/L)治疗后大豆苷元浓度较基线升高(P<；0.05)。卡那霉素(31.2+/-6.2vs.479+/-188nmol/L)和甲硝唑(56.0+/-27.9vs.414+/-212nmol/L)治疗后，二氢大豆苷元也有类似的增加(P<；0.05)。终止抗生素治疗后，异黄酮类药物浓度恢复到基线水平。与基线相比，包括对照在内的所有处理都减少了革兰氏(+)细菌的生长。总而言之，抗生素治疗导致食蟹猴血浆马酚浓度显著降低，并改变了血浆异黄酮型式。
To explore the importance of equol on health outcomes in future studies, it was necessary to develop a method to reduce equol production. Female monkeys (n = 22) fed a soy diet were treated twice daily with vehicle (control; n = 4), doxycycline (2.5 mg/kg; n = 4), metronidazole (125 mg/d; n = 3), kanamycin (1000 mg/d; n = 4), vancomycin (100 mg/d; n = 3) or kanamycin+vancomycin (n = 4). Plasma samples were collected 4 h postfeeding at baseline, after 4 wk of treatment and 8 wk after the end of treatment and analyzed for isoflavonoid concentrations. Fecal swabs were collected at baseline and at the end of antibiotic treatment for analysis of Gram(+) and Gram(-) bacterial growth. Equol concentrations were reduced (P < 0.05) compared with baseline by 80, 93, 98 and 99% after treatment with metronidazole (955 +/- 164 vs. 193 +/- 53 nmol/L), kanamycin (545 +/- 211 vs. 37.1 +/- 17.6 nmol/L), vancomycin (607 +/- 163 vs. 8.9 +/- 8.2 nmol/L) and kanamycin+vancomycin (721 +/- 169 vs. 17.4 +/- 17.3 nmol/L), respectively. Daidzein concentrations were increased (P < 0.05) compared with baseline by treatment with doxycycline (336 +/- 87 vs. 576 +/- 76 nmol/L), kanamycin (168 +/- 67 vs. 374 +/- 15 nmol/L), and kanamycin+vancomycin (166 +/- 35 vs. 384 +/- 78 nmol/L). Similar increases (P < 0.05) in dihydrodaidzein were observed after treatment with kanamycin (31.2 +/- 6.2 vs. 479 +/- 188 nmol/L) and metronidazole (56.0 +/- 27.9 vs. 414 +/- 212 nmol/L). Isoflavonoid concentrations returned to baseline values after antibiotic treatment was terminated. Gram(+) bacterial growth was reduced by all treatments, including Control, compared with baseline. In conclusion, treatment with antibiotics resulted in a marked reduction in plasma equol concentrations and altered plasma isoflavonoid patterns in cynomolgus monkeys.