Kainate administered to adult zebrafish causes seizures similar to those in rodent models
Kainate administered to adult zebrafish causes seizures similar to those in rodent models
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DOI：
10.1111/j.1460-9568.2011.07622.x
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发表时间：
2011-04-01
影响因子：
3.4
通讯作者：
Burgos, Javier S.
中科院分区：
文献类型：
--
作者：
Alfaro, Juan M.;Ripoll-Gomez, Jorge;Burgos, Javier S.
谷氨酸是脊椎动物中枢神经系统的主要兴奋性神经递质。由谷氨酸受体的过度刺激引起的兴奋性毒性，是包括癫痫在内的几种脑部疾病中神经元死亡的主要原因。我们在这里描述了如何在成年斑马鱼中通过给药kainate触发行为癫痫发作，并且与在啮齿动物模型中观察到的非常相似。Kainate诱导了一系列剂量依赖性的行为改变，最终导致clon样抽搐。DNQX（6,7-二硝基喹啉-2,3-二酮）剂量依赖性地抑制行为癫痫发作，而MK-801（一种非竞争性NMDA受体拮抗剂）的作用较小。Kainate可以触发成年斑马鱼的癫痫发作，因此该物种可以被认为是研究癫痫发作和随后的兴奋性毒性脑损伤的新模型。
Glutamate is the major excitatory neurotransmitter of the central nervous system in vertebrates. Excitotoxicity, caused by over-stimulation of the glutamate receptors, is a major cause of neuron death in several brain diseases, including epilepsy. We describe here how behavioural seizures can be triggered in adult zebrafish by the administration of kainate and are very similar to those observed in rodent models. Kainate induced a dose-dependent sequence of behavioural changes culminating in clonus-like convulsions. Behavioural seizures were suppressed by DNQX (6,7-dinitroquinoxaline-2,3-dione) dose-dependently, whilst MK-801 (a non-competitive NMDA receptor antagonist) had a lesser effect. Kainate triggers seizures in adult zebrafish, and thus this species can be considered as a new model for studying seizures and subsequent excitotoxic brain injury.