Implantable patches assembled with mesenchymal stem cells and gelatin/silk fibroin composite microspheres for the treatment of traumatic optic neuropathy
Implantable patches assembled with mesenchymal stem cells and gelatin/silk fibroin composite microspheres for the treatment of traumatic optic neuropathy
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DOI：
10.1016/j.apmt.2021.101278
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发表时间：
2021-11-30
影响因子：
8.3
通讯作者：
Nan，Kai-hui
中科院分区：
文献类型：
--
作者：
Wang，Jing-jie;Wang，Tian-zuo;Nan，Kai-hui
将间充质干细胞(MSCs)移植到外伤性视神经病变(TON)的损伤部位的治疗效果是一个关键但尚未解决的问题，这个问题的答案可能会促进Ton的创新疗法的发展，Ton是一种令人沮丧的疾病，没有有效的治疗方法。在这里，我们通过证明MSC移植到损伤部位能够在视网膜神经节细胞存活和轴突再生方面促进视神经再生，使用MSC@MS、由MSCs组装的可植入补片和集3D培养和无胰酶采集为一体的明胶/丝素复合微球，并可以增加移植MSCs的活体保留。相比之下，传统的MSC混悬液团注不足以产生显著的好处。我们还发现，在IGF-1刺激后，MSC@MS中的MSCs表现出优于单层培养的基因表达谱。在未来的研究中，MSC@MS中的微球可以进一步被利用来提供治疗货物，以提高MSCs的效力或与MSCs协同治疗TON，使MSC@MS成为为TON开发基于MSC的定制疗法的通用平台。
The therapeutic benefits of transplanting mesenchymal stem cells (MSCs) to the injury site of traumatic optic neuropathy (TON) represents a crucial, yet unresolved question, the answer to which may facilitate development of innovative therapies for TON, a frustrating disease with no effective treatment. Herein, we address this issue by showing that MSC transplantation to the injury site is able to promote optic nerve regeneration in terms of retinal ganglion cell survival and axon regeneration using MSC@MS, implantable patches assembled with MSCs and gelatin/silk fibroin composite microspheres, which integrate 3D culture and trypsin-free harvesting, and can increasein vivoretention of transplanted MSCs. In contrast, conventional bolus injection of MSC suspension is not potent enough to produce significant benefits. We also show MSCs in MSC@MS demonstrate superior gene expression profiles over monolayer culture after IGF-1 stimulation. In future study, microspheres in MSC@MS can further be harnessed to deliver therapeutic cargoes that boost the potency of MSCs or treat TON synergistically with MSCs, making MSC@MS a versatile platform to develop tailored MSC-based therapies for TON.