Phytochrome control of levels of mRNA complementary to plastid and nuclear genes of maize.
Phytochrome control of levels of mRNA complementary to plastid and nuclear genes of maize.
复制标题
光敏色素控制与玉米质体和核基因互补的 mRNA 水平。
DOI：
10.1104/pp.79.2.371
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发表时间：
1985
期刊：
影响因子：
7.4
通讯作者：
Lawrence Bogorad
中科院分区：
文献类型：
--
作者：
Yu S. Zhu;Shain D. Kung;Lawrence Bogorad
研究了光敏色素对玉米叶绿体和核基因转录的RNA水平的调控作用。红光、远红光或红光后远红光对玉米叶绿体基因互补核酮糖二磷酸羧基酶大亚基(RuBPCase)的相对数量的影响；光系统II的32千道类囊体膜三嗪除草剂结合B蛋白；Cf的α、β和epsilon亚基(1)；Cf的亚基III(0)；光系统I的反应中心蛋白A1和A2；光系统II的膜蛋白的另外两个光诱导基因(orf353和473)；一个未知的膜蛋白基因(UORF443)通过标记的DNA探针与叶片RNA的杂交进行了检测。用同样的方法研究了RuBPCase小亚基基因和捕光叶绿素a/b结合蛋白这两个核编码基因的转录本。与所有这些光诱导基因互补的RNA水平在红光短暂光照后3-6小时内显著增加。红光的刺激效应在很大程度上被随后的远红光照射所逆转。因此，在暗生长的玉米幼苗中，光敏色素控制了与某些叶绿体和核基因互补的mRNAs水平的增加。
The involvement of phytochrome in the control of the levels of RNA transcribed from maize plastid and nuclear genes was examined. The effects of illumination with red light, far-red light, or red light followed by far-red light on relative amounts of RNAs complementary to maize plastid genes for the large subunit of ribulose bisphosphate carboxylase (RuBPCase); the 32-kilodalton thylakoid membrane triazine herbicide binding B protein of photosystem II; the alpha, beta, and epsilon subunits of CF(1); subunit III (proton-translocating) of CF(0); the reaction center proteins A1 and A2 of photosystem I; two other light-induced genes for membrane proteins of photosystem II (ORFs 353 and 473); and one gene for an unidentified membrane protein (UORF 443) were measured by hybridization of labeled DNA probes to samples of leaf RNA. Transcripts of two nuclear-encoded genes, the genes for the small subunit of RuBPCase and the light-harvesting chlorophyll a/b binding protein, were studied in the same way. The levels of RNA complementary to all of these light-induced genes were significantly increased within 3 to 6 hours after brief illumination with red light. The stimulatory effects of red light were largely reversed by subsequent illumination with far-red light. It is concluded that phytochrome controls increases in the levels of mRNAs complementary to certain plastid and nuclear genes in dark-grown maize seedlings.