Histological distribution of class III alcohol dehydrogenase in human brain
Histological distribution of class III alcohol dehydrogenase in human brain
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DOI：
10.1016/s0006-8993(99)02201-5
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发表时间：
2000-01-03
期刊：
影响因子：
2.9
通讯作者：
Asano, G
中科院分区：
文献类型：
--
作者：
Mori, O;Haseba, T;Asano, G
通过免疫组织化学方法检查了 III 类乙醇脱氢酶 (ADH)（一种谷胱甘肽依赖性甲醛脱氢酶）和 I 类 ADH 在人脑中的分布。在小脑浦肯野细胞的树突和细胞质中观察到最强烈的 III 类 ADH 免疫染色。 IV层和V层分散的大脑皮质神经元以及一些海马锥体神经元也呈免疫阳性。 III 类 ADH 的神经元分布类似于缺氧性脑病患者中脆弱神经元的分布，鉴于浦肯野细胞中的强烈染色，提出了这种酶导致慢性酗酒者遭受缺氧和小脑变性的可能性。血管周围和室管膜下星形胶质细胞有助于维持脑细胞环境并将大脑与体循环和脑脊液隔离，也是 III 类 ADH积极的。由于该酶的底物包括内在毒性甲醛、20-羟基白细胞素 B4 的炎症中间体以及可能的乙醇，因此 III 类 ADH 免疫染色的分布表明该酶有助于保护大脑免受退化过程的影响。与室管膜细胞不同，室管膜下星形胶质细胞呈 III 类 ADH 阳性，这一发现表明这种酶可能有助于区分星形胶质细胞和室管膜细胞。 (C) 2000 Elsevier Science B.V. 保留所有权利。
The distributions of class III alcohol dehydrogenase (ADH), a glutathione-dependent formaldehyde dehydrogenase, and class I ADH in the human brain were examined immunohistochemically. The most intense immunostaining of class III ADH was observed in the dendrites and cytoplasm of cerebellar Purkinje cells. Scattered cerebral cortical neurons in layers IV and V, and some hippocampal pyramidal neurons were also immunopositive. The neuronal distribution of class III ADH resembled that of the vulnerable neurons in patients with hypoxic encephalopathy, which in view of the intense staining in the Purkinje cells, raises the possibility that this enzyme contributes to the hypoxia and cerebellar degeneration suffered by chronic alcoholics, Perivascular and subependymal astrocytes, which contribute to the maintenance of the cerebral cellular milieu and isolate the brain from the systemic circulation and cerebrospinal fluid, were also class III ADH positive. As the substrates of this enzyme include intrinsic toxic formaldehyde, inflammatory intermediate of 20-hydroxy-leukoteiene B4, and possibly ethanol, the distribution of class III ADH immunostaining indicates this enzyme contributes to the defence of the brain against degenerative processes. The finding that, unlike ependymal cells, subependymal astrocytes were class III ADH positive, suggests this enzyme may be useful for differentiating astrocytes and ependymal cells. (C) 2000 Elsevier Science B.V. All rights reserved.