Photothermal temperature control of a chemical reaction on a microchip using an infrared diode laser
Photothermal temperature control of a chemical reaction on a microchip using an infrared diode laser
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DOI：
10.1021/ac010318p
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发表时间：
2001-08-15
影响因子：
7.4
通讯作者：
Kitamori, T
中科院分区：
文献类型：
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作者：
Slyadnev, MN;Tanaka, Y;Kitamori, T
我们已经证明了一种基于光热效应的红外激光加热溶剂的小型化装置，能够在流动条件下在微芯片上快速和局部控制酶促反应。采用非接触式光谱温度传感技术，测量了温度动态和空间分布，并与数值模拟分析结果进行了比较。该装置的加热和冷却速度分别为67度/秒和53度/秒，比传统系统快30倍，比电热小型化热循环器快3-6倍。与现有的基于芯片的电热加热系统相比，红外激光介导的加热器的特点是加热体积仅为5 nL。直接加热具有极小热容量的样品使我们获得了快速的加热速率，并且通过热量传递到玻璃基板的有效散热导致了快速的冷却速率。获得了停留时间短于0.5 s的可重复性温度水平。利用红外激光的周期性光热加热，成功地控制了酶在芯片上的反应，时间分辨率为0.6 s。红外二极管激光器结构紧凑，非常适合微型化系统设计。我们的工作为需要快速温度控制的各种化学过程的芯片格式集成提供了基础。
We have demonstrated that a miniaturized device with IR laser heating of the solvents based on a photothermal effect, is capable of fast and localized control of an enzymatic reaction on a microchip under flow conditions. Using noncontact spectroscopic temperature-sensing techniques, we measured temperature dynamics and spatial distribution and compared the measurements with results of numerical simulation analysis. The device was operated at ultrafast heating and cooling rates of 67 and 53 degreesC/s, respectively, which is 30 times faster than conventional systems and 3-6 times faster than electrothermal miniaturized thermocyclers. The IR laser-mediated heater is characterized by a significantly reduced heated volume of only 5 nL, compared to existing chip-based systems with electrothermal heating. Direct heating of a sample with extremely small heat capacity led us to a fast heating rate, and efficient heat removal through heat transfer to the glass substrate resulted in a fast cooling rate. Reproducible temperature levels with dwell times shorter than 0.5 s were achieved. The enzyme reaction on a chip was successfully controlled with 0.6-s time resolution, using periodic photothermal heating by IR laser. The IR diode laser is compact and thus suits well the miniaturized system design. Our work gives the basis for integration in a chip format of a variety of chemical processes that require fast temperature control.