FGF-7 Dictates Osteocyte Cell Processes Through Beta-Catenin Transduction.
FGF-7 Dictates Osteocyte Cell Processes Through Beta-Catenin Transduction.
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FGF-7 通过 β-连环蛋白转导控制骨细胞过程
DOI：
10.1038/s41598-018-33247-8
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发表时间：
2018-10-04
影响因子：
4.6
通讯作者：
Ye L
中科院分区：
文献类型：
--
作者：
Liu XY;Li X;Bai MR;Chen X;Wang CL;Xie J;Ye L
众所周知，骨细胞通过间隙连接相互通信，并且连接蛋白43（Cx43）显示出其在间隙连接中的巨大潜力，其贡献使得能够传递小分子并以自分泌/旁分泌方式操作。成纤维细胞生长因子（Fibroblast growth factors，FGF）在新骨形成和成人骨重建中起重要作用，FGF信号转导受精确的时空调控。然而，FGF 7对骨细胞过程的影响还没有得到很好的阐明。在这项研究中，我们的目的是研究的影响，FGF 7对骨细胞的细胞过程的特点表达的Cx43，并揭示了潜在的机制，调节这一细胞过程。我们首先发现，在骨细胞系（MLO-Y 4）和骨组织中，FGF 7的mRNA水平相对于其他FGF家族成员更高。我们随后证实，FGF 7可以增加骨细胞中Cx43的表达，并以骨细胞之间的间隙连接的形式促进细胞过程。这种调节是由于FGF 7诱导的细胞质积累和由此产生的β-连环蛋白的核转位。我们的研究结果有助于我们进一步了解FGF 7对骨细胞行为和骨生理甚至病理的重要性。
It is well recognized that osteocytes communicate with each other via gap junctions and that connxin43 (Cx43) shows its great potential in gap junction for the contribution enabling transmission of small molecules and operating in an autocrine/a paracrine manner. Fibroblast growth factors (FGFs) play significant roles in new bone formation and adult bone remodeling, and FGF signaling is regulated by the precise spatiotemporal approaches. However, the influence of FGF7 on osteocyte cell processes is not well elucidated. In this study, we aimed to examine the impact of FGF7 on osteocyte cell processes by characterizing the expression of Cx43 and to reveal the underlying mechanism regulating this cell process. We first found that the mRNA level of FGF7 was higher relative to other FGF family members both in osteocytes cell line (MLO-Y4) and bone tissue. We then demonstrated that FGF7 could increase the expression of Cx43 in osteocytes and promote the cell processes in the form of gap junctions between osteocytes. This modulation was due to the FGF7-induced cytoplasmic accumulation and resultant nuclear translocation of β-catenin. Our results could help us to further understand the importance of FGF7 on bone cell behavior and bone physiology and even pathology.
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影响因子：
2.8
作者：
Loiselle, Alayna E.;Paul, Emmanuel M.;Lewis, Gregory S.;Donahue, Henry J.
通讯作者：
Donahue, Henry J.
影响因子：
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作者：
Schiller, PC;D'Ippolito, G;Howard, GA
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影响因子：
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作者：
Schiller, PC;D'ippolito, G;Howard, GA
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影响因子：
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影响因子：
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作者：
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