Responses to Sulfated Steroids of Female Mouse Vomeronasal Sensory Neurons
Responses to Sulfated Steroids of Female Mouse Vomeronasal Sensory Neurons
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DOI：
10.1093/chemse/bjs068
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发表时间：
2012-11-01
期刊：
影响因子：
3.5
通讯作者：
Menini, Anna
中科院分区：
文献类型：
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作者：
Celsi, Fulvio;D'Errico, Anna;Menini, Anna
啮齿动物犁鼻器在许多社会行为中发挥着重要作用。使用荧光染料 4 的钙成像技术，我们测量了对从雌性小鼠犁鼻器分离的神经元应用硫酸化类固醇引起的细胞内钙浓度变化。我们发现来自雄激素、雌激素、孕烯醇酮和糖皮质激素家族的 10 种硫酸化类固醇的混合物可在 71% 的神经元中诱导钙反应。此外，31％的神经元对由 3 种糖皮质激素衍生化合物组成的混合物有反应，28％的神经元对由 3 种孕烯醇酮衍生化合物组成的混合物有反应。免疫组织化学显示，对硫酸类固醇做出反应的神经元表达磷酸二酯酶 4A，这是表达 V1R 受体的顶端神经元的特异性标记物。对一种混合物做出反应的神经元都不会同时对另一种混合物作出反应，这表明反应的特异性。一些神经元对高浓度测试的糖皮质激素衍生混合物中超过 1 种单独的成分有反应，这表明这些神经元经过广泛调整，尽管它们仍然表现出很强的特异性，对高浓度的无效化合物仍然没有反应。
The rodent vomeronasal organ plays an important role in many social behaviors. Using the calcium imaging technique with the dye fluo-4 we measured intracellular calcium concentration changes induced by the application of sulfated steroids to neurons isolated from the vomeronasal organ of female mice. We found that a mix of 10 sulfated steroids from the androgen, estrogen, pregnanolone, and glucocorticoid families induced a calcium response in 71% of neurons. Moreover, 31% of the neurons responded to a mix composed of 3 glucocorticoid-derived compounds, and 28% responded to a mix composed of 3 pregnanolone-derived compounds. Immunohistochemistry showed that neurons responding to sulfated steroids expressed phosphodiesterase 4A, a marker specific for apical neurons expressing V1R receptors. None of the neuron that responded to 1 mix responded also to the other, indicating a specificity of the responses. Some neurons responded to more than 1 individual component of the glucocorticoid-derived mix tested at high concentration, suggesting that these neurons are broadly tuned, although they still displayed strong specificity, remaining unresponsive to high concentrations of the ineffective compounds.