Localization of epidermal growth factor precursor in tooth and lung during embryonic mouse development.
Localization of epidermal growth factor precursor in tooth and lung during embryonic mouse development.
复制标题
小鼠胚胎发育过程中表皮生长因子前体在牙齿和肺中的定位。
DOI：
10.1016/0012-1606(89)90114-0
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发表时间：
1989
影响因子：
2.7
通讯作者：
Slavkin,HC
中科院分区：
文献类型：
--
作者：
Snead,ML;Luo,W;Oliver,P;Nakamura,M;Don-Wheeler,G;Bessem,C;Bell,GI;Rall,LB;Slavkin,HC
小鼠表皮生长因子（EGF）前体是一种含有1217个氨基酸的蛋白质，包含成熟的EGF（氨基酸残基977-1029）和8个EGF样重复序列。虽然在成年雄性小鼠的下颌下腺中发现了最高水平的EGF，但原位杂交研究和mRNA分析的结果表明，EGF前体mRNA在几种成年小鼠组织中合成，包括肺和门牙。为了确定EGF前体基因的表达是否与胚胎牙齿或肺器官发生的发育过程有关，我们利用与EGF前体1070-1081氨基酸对应的正义和反义寡脱氧核糖核苷酸探针，通过原位杂交来定位EGF前体mRNA合成的细胞位点。针对前体氨基酸残基348-691的抗体用于免疫检测技术，以鉴定EGF前体蛋白或加工衍生物。与先前报道的小鼠胚胎组织不合成EGF前体mRNA相反，我们发现EGF前体mRNA存在于与胚胎牙齿和肺器官相关的外胚层、中胚层和外间充质来源的细胞簇中。此外，EGF前体共有的表位在胚胎小鼠牙齿和肺器官的上皮和间充质组织中都有免疫定位。这些结果表明，EGF前体和/或包含在前体分子中的基序，包括成熟的EGF，可能在器官发生的上皮-间质相互作用中发挥指导或许可作用。
The murine epidermal growth factor (EGF) precursor is a 1217 amino acid protein which contains mature EGF (amino acid residues 977–1029) as well as eight EGF-like repeats. Although the highest levels of EGF are found in the adult male mouse submandibular gland, the results ofin situhybridization studies and mRNA analyses suggest that EGF precursor mRNA is synthesized in several adult mouse tissues including the lung and the incisor. To determine if EGF precursor gene expression is intrinsic to the developmental program for either embryonic tooth or lung organogenesis, sense and antisense oligodeoxyribonucleotide probes corresponding to amino acids 1070–1081 of the precursor were used to localize cellular sites of synthesis of EGF precursor mRNA byin situhybridization. Antibodies directed against amino acid residues 348–691 of the precursor were used in immunodetection techniques to identify either EGF precursor protein or processed derivatives. In contrast to earlier reports indicating that embryonic mouse tissues do not synthesize EGF precursor mRNA, we found that EGF precursor mRNA is present in clusters of ectoderm-, mesoderm-, and ectomesenchyme-derived cells associated with embryonic teeth and lung organs. Moreover, epitopes common to the EGF precursor were immunolocalized in both the epithelial and mesenchymal tissues of embryonic mouse tooth and lung organs. These results suggest that the EGF precursor and/or motifs contained within the precursor molecule, including mature EGF, may play an instructive or permissive role in epithelial-mesenchymal interactions pursuant to organogenesis.